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1、南方農(nóng)業(yè)學(xué)報(bào)JOuRNAL0FSOUTHERNAGRlCUITuRE2012，43(2)：155—159ISSN2095—119l：CODENNNXAABhttp：／／ww．nfnyxb．comDOI：10．3969／j：issn．2095一l191．2012．02．155Optimizationofmediaforjn訂frDrootinductionindifferentgenotypeS0tSWeetS0r2nUmuvIoGEYu—hong，XIEHong—kai，HANJia—yu(AgriculturalCollege，GuangxiUniversity，Nanni
2、ng530005，China)Abstract：【0bjectiVe】111epresentexperimentwasconductedtooptimizetherootregenerationsysteminsweetso唱huminordertodeve】opaneH’ectivegenetictransf0咖ationpmtoc01．【Method】Difkrentbasicmedia(MS，1／2Ms)anddif玷rentconcentmtionsofgmwthhoHnones(IBA，NAAandABT)wereevaluatedandtheiref玷ctsonv
3、ariousfactorssuchasrootqua】ity，motingra￡e，rootjng￡ime，gro講hofrootedplant】ets，e把．，weredete肋inedtoobtajnanoptimumroo“ngmedium．【Result】Theoptimumrootingmediumforthesweetsor曲umvarietyBT3and【JT3werefoundasl／2MS+IBA(2．0mg，L)and1／2MS+IBA(3．0mg／L)，respectively．TheI℃generatedmotswerethick，andthemote
4、dplantletsshowedvigorousgrowth．Therootingratesvariedintwovarieties．【conclusion】Itisconcludedthatbestqualityofrootscanberegeneratedonshootsusingl／2MSmedium，supplementedwith2．0or3．0mg／LIBAforvarietyBT3and【』3，respectively．Keywords：sweetsorghum；plantletmoting；optimization；plantho珊one；basicmediu
5、mCLCnumber：S514．035．3Doc唧entcode：AArticle：2095—1191(2012)02—0155—05甜高梁組培苗生根培養(yǎng)的優(yōu)化葛玉紅，謝鴻鍇，韓佳宇(廣西大學(xué)農(nóng)學(xué)院，南寧530005)摘要：【目的】優(yōu)化能源作物甜高粱組培苗的生根培養(yǎng)體系，為遺傳轉(zhuǎn)化奠定基礎(chǔ)?！痉椒ā坷貌煌九囵B(yǎng)基(MS、1／2MS)對組培苗生根的影響進(jìn)行篩選試驗(yàn)，得到較有利于生根的基本培養(yǎng)基；在最佳培養(yǎng)基中添加不同濃度的植物激素(IBA、NAA、ABT)，綜合根的質(zhì)量、生根率、生根時(shí)間、生根苗的長勢等篩選最佳生根培養(yǎng)基配方?！窘Y(jié)果】篩選出最佳生根培養(yǎng)基組合：北甜三號(BT
6、3)為1／2Ms+2．Omg，LIBA，遼甜三號(LT3)為1／2MS+3．0mg／LIBA，且根粗、苗壯，兩品種的生根率存在差異。此外，未加激素的Ms和1／2Ms培養(yǎng)基均無組培苗生根?！窘Y(jié)論】在1／2Ms上添加2．om璣IBA或3．0m∥LIBA分別為北甜三號、遼甜三號組培苗生根的最佳途徑。關(guān)鍵詞：甜高粱；組培苗生根；優(yōu)化；植物激素；基本培養(yǎng)基Sweetsorghumiswjdelyconsjderedasoneofthemostoutstandingenergycrop．Itsphotosyntheticefficiencyis2or3“meshigherthanthat
7、ofwheatandmanyothercrops．ItcanbeusedasagI．a(chǎn)inaswellasasugar—energycrop．Butsofar，thereareverylesssweetsorghumVarietieswhichpossesssuperiorqualitytraits．Maqbooleta1．(2001)advocatedtheimprovementofsorghumusingbiotechn010百calt001sandtechniques．Transgenictech
