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1、第5卷第1期食品安全質(zhì)量檢測學(xué)報Vol.5No.12014年1月JournalofFoodSafetyandQualityJan.,2014一株多重耐藥副溶血性弧菌的耐藥分子機(jī)制研究*江艷華,姚琳,李風(fēng)鈴,翟毓秀,王聯(lián)珠(中國水產(chǎn)科學(xué)研究院黃海水產(chǎn)研究所,農(nóng)業(yè)部水產(chǎn)品質(zhì)量安全檢測與評價重點(diǎn)實(shí)驗(yàn)室,青島266071)摘要:目的從養(yǎng)殖半滑舌鰨腸道分離到一株副溶血性弧菌Vp1107,對該菌株進(jìn)行耐藥性及耐藥分子機(jī)制研究。方法通過K-B法進(jìn)行藥敏試驗(yàn),PCR擴(kuò)增及測序法對耐藥基因及整合子進(jìn)行檢測與分析。結(jié)果Vp110
2、7菌株耐受氨芐西林、阿莫西林、頭孢唑啉、四環(huán)素、土霉素和氯霉素,對頭孢呋辛鈉、鏈霉素、卡那霉素、萘啶酸、環(huán)丙沙星中度敏感,其多重耐藥系數(shù)為0.33。菌株攜帶blaTEM、strA、strB、tetB、tetM、catⅢ、intI1耐藥相關(guān)基因,1類整合子不含基因盒,gyrA和parC基因的喹諾酮類耐藥決定區(qū)未發(fā)生點(diǎn)突變。結(jié)論副溶血性弧菌Vp1107耐藥程度較為嚴(yán)重,其耐藥性主要由耐藥基因編碼的耐藥性酶類和外排泵作用引起,提示應(yīng)加強(qiáng)對副溶血性弧菌耐藥性的監(jiān)控及漁用藥物的管理。關(guān)鍵詞:副溶血性弧菌;多重耐藥性;耐
3、藥基因;整合子;喹諾酮耐藥決定區(qū)Studiesonmolecularmechanismofamulti-drugresistantVibrioparahaemolyticusstrain*JIANGYan-Hua,YAOLin,LIFeng-Ling,ZHAIYu-Xiu,WANGLian-Zhu(KeyLaboratoryofTestingandEvaluationforAquaticProductSafetyandQuality,MinistryofAgriculture,YellowSeaFisheri
4、esResearchInstitute,ChineseAcademyofFisherySciences,Qingdao266071,China)ABSTRACT:ObjectiveThedrugresistanceandthemolecularresistancemechanismofaVibrioparahae-molyticusstrainVp1107fromthegutofthehalf-smoothtonguesolewerestudied.MethodsThedrugresis-tancewaspe
5、rformedbyK-BmethodandtheresistancegenesandintegronsweredeterminedbyPCRamplifi-cationandDNAsequencing.ResultsVp1107wasresistanttoampicillin,amoxycillin,cephazolin,tetracyc-line,oxytetracyclineandchloramphenicol,andintermediate-sensitivetocefuroximesodium,str
6、eptomycin,ka-namycin,nalidixicacidandciprofloxacin.Themulti-drugresistanceindexofthisstrainwas0.33.blaTEM、strA、strB、tetB、tetM、catⅢ、intI1geneswerepresentinVp1107andclass1integronwasanemptyintegronwithoutanygenecassettes.Therewerenomutantswithinquinoloneresis
7、tance-determiningregionofgyrAandparCgenes.ConclusionThedrugresistanceofVp1107wasrelativelyseverewhichwasduetoinactivatedormodifiedenzymesandeffluxpumps,indicatingitisnecessarytostrengthenthemonitoringofdrugresistanceofV.parahaemolyticusandthemanagementofdru
8、gs’usage.KEYWORDS:Vibrioparahaemolyticus;multi-drugresistance;drugresistancegenes;integrons;quinoloneresistance-determiningregion基金項(xiàng)目:黃海水產(chǎn)研究所級基本科研業(yè)務(wù)經(jīng)費(fèi)(20603022012010)Fund:SupportedbySpecialScientificRe
