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1、中西醫(yī)結(jié)合肝病雜志2014年第24卷第4期·239·doi：10．3969／j．issn．1005-0264．2014．04．016丹參對(duì)暴發(fā)性肝衰竭大鼠肝損傷及肝再生的影響朱琥朱清靜武漢市醫(yī)療救治中心(湖北武漢，430023)摘要目的：探討丹參對(duì)暴發(fā)性肝衰竭(FHF)大鼠肝損傷及肝再生的影響。方法：Wistar大鼠隨機(jī)分為4組：正常對(duì)照組、FHF組、丹參組、促肝細(xì)胞生長素(PHGF)組。FHF、丹參組和PHGF組動(dòng)物模型采用TAA皮下注射，劑量按每kg體重600mg，2次，每次間隔24h，復(fù)制FHF動(dòng)物模型。丹參組和PHGF組動(dòng)物除皮下注射TAA外，于實(shí)驗(yàn)前3天至實(shí)驗(yàn)結(jié)束分別于皮下注射丹
2、參注射液1ml／100g和PHGF1ml／100g，正常對(duì)照組和FHF組大鼠同時(shí)皮下注射生理鹽水lml／100g。FHF組、丹參組和PHGF組，于第2次注射TAA后24h，隨機(jī)取大鼠各8只，腹主動(dòng)脈取血測肝功能。迅速取肝組織，用10％甲醛液固定，制成石蠟切片，檢測肝細(xì)胞有絲分裂指數(shù)(MI)和增殖細(xì)胞核抗原(PCNA)。結(jié)果：丹參具有降低FHF大鼠ALT、AST及TBil，顯著提高肝細(xì)胞MI和PCNA的作用(P<0．05，P<0．01)。結(jié)論：丹參具有改善FHF大鼠肝功能和促進(jìn)肝細(xì)胞增殖及再生的作用。關(guān)鍵詞暴發(fā)性肝衰竭；肝再生；增殖細(xì)胞核抗原；丹參注射液／藥理作用；大鼠EfectofRadi
3、xSalviaeMiltiorrhizaeonliverdamageandliverregenerationinthioacetamide．inducedacutehepaticfailureinratSZHUHu，ZHUQing-jing．WuhanMedicalTreatementCenter(WuhanHubei，430023)ChinaAbstractObjective：TostudytheefectofRadixSalviaeMiltiorrhizaeonliverdamageandliverregenerationinthioacet—amide·inducedacutehep
4、aticfailureinrats．Methods：Theratswererandomlydividedintonormalgroup，modelgroup，RadixSalviaeMiltiorrhizaegroupandlyophilizedbovinetransferfactorcontrolgroup．Medicationsweregivenlml／100grespectively3daysbeforehypodermicallyinjectedwiththioacetamide(TAA)onceaday．Theratswerehypodermicallyinjectedwithr
5、AA600mg·kg一twiceatsametimefortwodaystoinducethemodelofacutehepaticfailureratexceptthenormalgroupinjectedwiththe0．9％saltsolutionhypodermically．Forty—eighthourslater，thebloodwerecollectedtomeasureALT，AST，TBil，andatthesametime，theparametersofliverregenerationwereobservedasfollowing：livermitoticindexa
6、ndproliferationcellnuclerantigen．Results：RadixSalviaeMihiorrhizaecouldobviouslydecreaseALT，AST，TBil，improvedlivermitoticindexandpro—liferationcellnucierantigenofratswithacutehepaticfailure．Comparingwithmodelgroupandcontrolgroup，thereweresignifi—cantdiference(P<0．05orP<0．01)．Conclusion：RadixSalviae
7、Miltiorrhizaehasesistanceagainsttheratwithacutehe—paticfailure，improveliverregeneration．KeyWordsactueliverfailure；liverregeneration；proliferationcellnucierantigen；RadixSalviaeMihiorrhizae／pharma-cologicalefect；ra
