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1、河北農(nóng)業(yè)科學,2014,18(5):30—32JournalofHebeiAgriculturalSciences編輯杜曉東高同淚Inn脅刪’迫]L!]_對金鉆蔓綠絨耐熱性的影響孑J、少興,延禱(山西農(nóng)業(yè)大學林學院,山西太谷030801)摘要:為了明確金鉆蔓綠絨對高溫環(huán)境的忍耐能力,以3~5個葉片的金鉆蔓綠絨扦插苗為試材,以室溫為對照,研究了高溫(35、40和45℃)脅迫不同時間‘(8、16、24、32和40h)對金鉆蔓綠絨葉片相對電導率和丙二醛含量的影響。結(jié)果表明:葉片相對電導率隨脅迫溫度的升高而逐漸降
2、低、隨脅迫時間的延長而逐漸上升,其中35℃所有脅迫處理的葉片相對電導率均顯著>其CK,脅迫溫度升高后葉片相對電導率反而顯著下降甚至與其CK差異不顯著,說明高溫脅迫導致葉片細胞膜受害程度加深,溫度過高時細胞甚至解體而失去活性;丙二醛含量隨脅迫溫度的升高和脅迫時間的延長均持續(xù)增加,除35cI=脅迫8h處理的葉片丙二醛含量顯著<其CK外,其他高溫處理的丙二醛含量均顯著>其CK。植物所處的環(huán)境溫度越高、時間越長,其葉片細胞受到的生理傷害就明顯越重,綜合分析高溫脅迫對葉片相對電導率和丙二醛含量的影響,認為金鉆蔓綠絨
3、可以勉強忍耐35℃高溫8h。關鍵詞:金鉆蔓綠絨;高溫脅迫;耐熱性;葉片;相對電導率;丙二醛含量中圖分類號:$682.36文獻標識碼:A文章編號:1008—1631(2014)05-0030-03EfectofHighTemperatureStressontheHeatResistanceofDiamondphilodendronSUNShao—xing,YAOYan—tao(CollegeofForestry,ShanxiAgriculturalUniversity,Taigu030801,China)A
4、bstract:InordertoclarifytheenduranceofDiamondphilodendrontohightemperatureenvironment,usingcuttingseedlingsofDiamondphilodendronwith3—5bladesasthetestmaterial,theroomtemperatureascontrol,theeffectsofhightemperature(35,40and45oC)stressatdifferenttimes(8,16
5、,24,32and40h)ontherelativeconductivityandMDAcontentofDiamondphilodendronleaveswerestudied.Theresultsshowedthattheleafrelativeconductivitywasdecreasedgraduallywiththeelevationofstresstemperatureandincreasedgraduallywithprolongingofstresstime,theleafrelativ
6、econductivityofallstresstreatmentsat35oCweresignificantlyhigherthanthatofCK,whenthestresstemperaturerises,leafrelativeconductivitywassignificantlydecreasedorevenhadnosignificantdifferenceswithCK,itindicatedthathightemperaturestresscauseddeepenofcellmembra
7、nedamagedegree,whenthetemperatureistoohigh,cellwasdisintegratedandlostactivity.MDAcontentwasincreasedwiththeelevationofstresstemperatureandprolongingofstresstime,MDAcontentofleavesat35oC8hwassignificantlylowerthanthatofCK,andthatofotherhightemperaturetrea
8、tmentswasremarkablygreaterthanCK.Theplantenvironmenttemperatureishigher,thetimeislonger,thephysiologicalinjuryofitsleafcellsissignificantlymoreweight.Comprehensiveanalysisofeffectsofhightemperaturestressonleafrelati