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1、SRG基因的克隆及原核表達(dá)物質(zhì)分解清除.如果Dc內(nèi)吞壞死物質(zhì)過(guò)多,往往不能將其充分溶解吸收,這亦可導(dǎo)致DC的壞死或凋亡.以上不同培養(yǎng)時(shí)問(wèn)的Dc在透射電鏡下超微結(jié)構(gòu)和功能狀態(tài)的表現(xiàn),證實(shí)利用連續(xù)貼壁法分離培養(yǎng)的細(xì)胞,具有Dc典型的結(jié)構(gòu)與多種功能表現(xiàn).【參考文獻(xiàn)】【1]BanchereauJ,SteinmanRM.Dendriticcellsandthecontrolofim—mumty[J].Nature,1998,392:245～250.[2]NishiokaY,HuaW,NishimuraN,eta1.Geneticmedificatonofdendriticcellsandits
2、applicationforcancerimmunotherapy[J].JMedInvest,2002,49(1/2):1一l7.【3]lna~aK,InabaM,RomaniN,eta1.Generationoflargenumbersofdendriticcellsfrommousebonemarrowculturessupplementedwithgranulocyte/macrophagecolony—stimulatingfactor[J].JExpMed.1992.176(6):1693—1702.SRG基因的克隆及原核表達(dá)閏露',高萍,栗艷,魚(yú)兵Cloningandp
3、rokoryoticexpressionofSRGMODERNONCOLOGY.Mar.2007,VOI.1.SailustoF,LanzavecchiaA.Eficientpresentationofsolubleantigenbyculturedhumandendriticcellsismaintianedbygranuloeyte/macrophagecolony—?stimulatingfactorplusintedeukia'-4anddownregulatedbytumornecrosisfactor[J].JExpMed,1994,179(4):1109～1117.Ko
4、idoS,HaraE.HommaS.eta1.Dendriticcellsfusedwithallo.geneiceoloreetalcancercelllinepresentmultiplecolorcctalcancer—specificantigensandinduceantitumorimmunity~instautolo-goustumorcells[J].ClinCancerRes,2005,11(21):7891～790o.張紅梅,張利旺,賈軍,等.腫瘤患者自體血漿誘導(dǎo)樹(shù)突狀細(xì)胞的實(shí)驗(yàn)研究[J].現(xiàn)代腫瘤醫(yī)學(xué),2005,13(6):740～743.張利旺,張紅梅,賈軍,
5、等.以AFP為靶點(diǎn)的肝癌樹(shù)突狀細(xì)胞免疫治療的實(shí)驗(yàn)研究[J].現(xiàn)代腫瘤醫(yī)學(xué),2005,13(6):736—739.YANLu,GAOPing,LIYan,YUBing'DepartmentofPharmacy,CenterofRehabilitation,ngHospital,￡FourthMilitaryMedicalUniversity,Xitm710033;DepartmentObstetricsandGynecology,TangduHospital,FourthMilitaryMedicalUniversity,Xi"an710038;'OrthopedicsOncology
6、InstituteofChinesePLA,TangduHospital,theFourthMilitaryMedicalUniversity,Xitm710038,China.【Abstract】0bjective:Toclone,expressandidentifyhumanSRGgene.Methods:TotalRNAwasextractedfromhumanosteosarcomacellsandthefu11lengthcDNAofSRGwasobtainedbyRT—PCR.TheSRGgenewasclonedin.topGEM—T—Easyvectorandsequ
7、enced.ThenthegenewasinsertedtoBamHIandSalIsiteofpET一28(a+)expressionvectortoconstructtheexpressionvectorwhichwastransformedintoE.coilBL21.Aftertl1etransformedbacteriawereinducedatIPTGfor2—6htheexpressedproteinwasanalyzedbySDS—PAGE
