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《抗氧化活性測定方法》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、抗氧化方法一、DeterminationofSuperoxideAnionScavengingAbility（超氧陰離子清除能力）1、determinedbyaCLmethodinthepyrogallol-luminolsystemonaBPCLUltra-weakluminescenceanalyzer。（焦性沒食子酸-發(fā)光胺，熒光檢測）2、步驟：10mLsample(不同濃度)+50mL焦棓酸(6.25*10-4mol/L)+0.94mLofamixturecontainingluminol(0.05mol/L)andcarbonatebuffer(pH10.2)（發(fā)
2、光胺用pH10.2碳酸鹽緩沖液配成0.05mol/L）3、Hi-V,800;Kv,-1;thespectralrangeofCL（波長范圍）180-800nm;溫度：30°C.總時間：300S，每2S讀數(shù)一次。4、對照：不加樣品（樣品用水代替）?？瞻撞患咏箺斔幔ㄓ脕碚{(diào)零）。二、DeterminationofScavengingAbilityonHydroxideRadicals（羥基自由基清除能力）1、CuSO4-Phen-Vc-H2O2CLsystem（1mL體系）2、50mLofsamplesolution（樣品液）+50mLofa1.0mmol/LCuSO4solut
3、ion（CuSO4溶液）+50mLofa1mmol/L1,10-phenanthrolinesolution（鄰二氮菲溶液）+700mLofa0.05mol/Lboratebuffer(pH9.0)（硼酸緩沖液）+100mLofa1mmol/Lascorbatesolution+50mLofa0.15%H2O2solution3、總時間400S，間隔3S。Hi-V,800;Kv,-1;thespectralrangeofCL（波長范圍）180-800nm;溫度：30°C.4、陽性對照：不加樣品（樣品用水代替）。空白不加H2O2（用來調(diào)零）。三、Determinationof
4、ScavengingEffectonHydrogenPeroxide（過氧化氫清除能力）1、luminol-H2O2system2、Theluminescentreactionwasinitiatedbymanuallyadding1mLofasolutioncontaining0.15mol/Lhydrogenperoxideand0.1mol/Lluminolperliterofcarbonatebuffer(0.05mol/L,pH9.4).Lightemissionvstimewasrecordedfor3minat2sintervals.步驟：0.15mol/L的
5、過氧化氫+0.1mol/L發(fā)光胺（用pH9.4的碳酸緩沖液配），總體系1ml.3、BPCLUltra-weakluminescenceanalyzer，Hi-V,800;Kv,-1;thespectralrangeofCL（波長范圍）180-800nm;溫度：30°C.4、Thecontrolwasperformedinthesamemannerinthemixturewithoutthesamplesolution,andthebackgroundwasdetectedwithoutH2O2addition。三、DeterminationofPreventingDNADa
6、mageEffect（DNA損傷）1、CuSO4-Phen-Vc-H2O2-DNACLsystem.2、步驟：Copperand1,10-phenanthrolinewerepremixedin0.1mol/LNaOAc/HOAc(pH5.5)buffer。3mg/mLDNAwasincubatedwithaphen-Cusolution.步驟：800mLofphen-Cu/DNAsolution+100mLof4.2*10-3mol/Lascorbate+200mLof6%H2O2+wereaddedwithoutintervaltoa100mLsamplesolutio
7、ntogiveafinalvolumeof1.2mL.（總體系1.2mL）3、ThekineticcurveofCLproducedinthephen/Cu/H2O2/ascorbatesystemwasimmediatelyrecorded.(Thecontrolwasperformedinthesamemannerinthemixturewithoutthesamplesolution,andthebackgroundwasdetectedwithoutH2O2addition.)4、1.0mmol/LCuSO
