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《自身免疫性感音神經(jīng)性聾基因治療的實驗研究（experimental study of gene therapy for autoimmune sensorineural hearing loss）》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫。
1、自身免疫性感音神經(jīng)性聾基因治療的實驗研究（Experimentalstudyofgenetherapyforautoimmunesensorineuralhearingloss）ExperimentalstudyofgenetherapyforautoimmunesensorineuralhearinglossPublished:2II.-9-.I4.4...IViews:I.4source:ChinaofCaiWenjun,TanChangqiang,LiYujin,HuangandLee,theFirstAff
2、iliatedHospitalofNanjingMedicalUniversityDepartmentofOtolaryngology&CreamotolaryngologyResearchOffice,JiangsuNanjing2III2.;NanjingUniversityofTechnologyChemicalcenter,Jiangsu,Nanjing[Abstract]Objective:TostudytheFasligand(FasILigand),whichisarecombinantreplica
3、tiondeficientadenovirusvector(2IIII.,FasL)GeneandleukocyteAbstract-interleukinII(ILIII)genetherapyforexperimentalautoimmunesensorineuralhearingloss.Methods:usingthesameinnereartissueantigenofFreundadjuvantinguineapigs,animalmodelcausedbyautoimmunesensorineural
4、hearinglossof2.,accordingtothematchingdesignwillbedividedinto4groups,throughthescalatympanimicroinjection,AgroupinjectedwithFasLgeneadenovirus(AdIFasL)groupwereinjectedwithadenoviruscarryingILIIIgene(AdIILIII)group,B,C(simpleinjectionofadenoviruscarryinggreenf
5、luorescentprotein,AdIGFP)groupwereinjectedwithphosphatebufferequivalent.GeneD.Auditorybrainstemevokedpotential,D(AB.)test,afterthetemporalboneparaffinsectionHEstainingandlightmicroscopeobservationofeachgroupwereobserved,twoearsunderwentspiralligamentandbasemen
6、tmembraneintransmissionelectronmicroscope.Eachgroupof3(9ears)receivedimmunofluorescenceandenzymeimmunohistochemicaltesttodetecttheexpressionofgeneproductsandadenovirustransfection.Results:immunohistochemistryshowedthattheadenoviruscarryingpurposegenecanbetrans
7、fectedwithstriavascularisandspiralligament,Cortiorgan,spiralganglion,cochlearvesselsandcochlearbonewallandotherpartsofthecell,andproducesthecorrespondingproteinproduct(ILIIIandFasL).ThemeancontrastofAB.IIIwavethresholdshowedthattheAgroupandtheBgroupweresignifi
8、cantlylowerthanthoseintheCgroupandtheDgroup.TheinflammatoryresponseoftheinnereartissuewasalsosignificantlylessthanthatoftheCgroup.Conclusion:therecombinantadenoviruscancarrythegene
