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1、冰片與順鉑聯(lián)用對Eca109（DDPr）細(xì)胞增殖、細(xì)胞周期和凋亡的影響戴翠萍，張徐寧，薛彩萍，季寧東[基金項(xiàng)目]：江蘇省淮安市科技支撐計(jì)劃項(xiàng)目（HAS2009002－2）；江蘇省中醫(yī)藥局科技項(xiàng)目（LB09077）*通訊作者：E-mail：hywxjnd1@163.com(淮陰衛(wèi)生高等職業(yè)技術(shù)學(xué)校，淮安市消化道腫瘤重點(diǎn)實(shí)驗(yàn)室，江蘇淮安223300)[摘要]目的：探討冰片與順鉑(cisplatin，DDP)聯(lián)用對耐DDP的食管癌細(xì)胞Eca109(DDPr)增殖、細(xì)胞周期和凋亡的影響。方法：采用遞增藥物質(zhì)量濃度持續(xù)作用誘導(dǎo)法建立耐DDP
2、的食管癌細(xì)胞Eca109(DDPr)。冰片聯(lián)合DDP作用于Eca109(DDPr)細(xì)胞，MTT法檢測Eca109(DDPr)細(xì)胞增殖情況；流式細(xì)胞術(shù)檢測細(xì)胞周期及細(xì)胞凋亡。結(jié)果：遞增藥物質(zhì)量濃度持續(xù)作用誘導(dǎo)6個(gè)月后，細(xì)胞可以在含1μg/mlDDP的培養(yǎng)液中正常生長，其對DDP的耐藥指數(shù)為15.886，所得細(xì)胞命名為耐DDP的食管癌細(xì)胞Eca109(DDPr)。單用冰片濃度≤1.560μg/ml時(shí)，對Eca109(DDPr)細(xì)胞無明顯抑制作用(P＞0.05)，但與DDP1μg/ml合用能顯著促進(jìn)DDP抑制Eca109(DDPr)細(xì)胞
3、的增殖（P＜0.05），并促進(jìn)DDP誘導(dǎo)Eca109(DDPr)細(xì)胞周期改變、細(xì)胞凋亡增加。細(xì)胞周期表現(xiàn)為G1期細(xì)胞明顯增多（P＜0.05），S期細(xì)胞、G2期細(xì)胞明顯減少（P＜0.05）；細(xì)胞的凋亡率明顯增加（P＜0.05）。結(jié)論：冰片促進(jìn)DDP抑制Eca109(DDPr)細(xì)胞增殖可能與改變Eca109(DDPr)細(xì)胞周期、增加細(xì)胞凋亡有關(guān)。[關(guān)鍵詞]冰片；順鉑；Eca109(DDPr)；細(xì)胞增殖；細(xì)胞周期；細(xì)胞凋亡[中圖分類號(hào)]R735.1[文獻(xiàn)標(biāo)識(shí)碼]AEffectsofBorneolcombinedwithcisplatin
4、onCellProliferationandCellCycleandApoptosisinHumanEsophagealCarcinomaCelllineEca109(DDPr)DAICui-ping，ZHANGXu-ning，XUECai-ping，JINing-dong（HuaiyinAdvancedVocationalandTechnicalHealthSchool,HuaianKeyLaboratoryoftheTumorofDigestiveSystem,Huaian223300,China）[Abstract]Obje
5、ctive：Toexploretheeffectsofborneolcombinedwithcisplatin(DDP)onCellproliferationandcellcycleandapoptosisinhumanEsophagealCarcinomacelllineEca109(DDPr).Methods：ThehumanesophagealcarcinomacelllineEca109wasexposedtocontinuousstepwise-increasedconcentrationofDDP.Drug-sensi
6、tivitywasmeasuredbyMTT.Thecellcycleandapoptosisofcellsweredeterminedbyflowcytometry.Results：After6monthsstepwisedrugexposure,thecellcouldnormallygrowandpropagateinthemediumwith1μg/mlofDDP,Itexhibitedaresistanceindexof15.886againstDDP.ThecelllinewasnamedEca109(DDPr).MT
7、Tassayshowedthatborneolattheconcentrationnomorethan1.560μg/mlhadnoobviousinhibitiontoEca109(DDPr)cells(P＞0.05),butitcouldsignificantlypromoteDDP(1μg/ml)toinhibittheproliferationofEca109(DDPr)cells（P＜0.05）.ComparedwithusingDDPalone,BorneolcombinedwithDDPcouldchangethec
8、ellcycle，significantlyincreasethenumberofcellsinG1stage（P＜0.05）anddecreasethenumberofcellsinSstageandG2stage（P＜0.05）,andobvi
