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1、見代腫瘤醫(yī)學(xué)2014年2月第22卷第2期MODERNONCOLOGY,F(xiàn)eb20f4,VOL.22,NO02·265B一欖香烯聯(lián)合順鉑對宮頸癌SiHa細胞增殖和凋亡的影響馬力天,任秦有,史恒軍,閆平。,魏忠海。,李棟棟,宿仕民,周艷,林艷云,鄭瑾Efectof13一ElemenecombinedwithDDPontheproliferationandapoptosisofSiHacelMaLitian一,RenQinyou,ShiHengjun,YanPing。,WeiZhonghai。,LiDongdong,SuShimin,ZhouYan,LinYanyun
2、,ZhengJin’DepartmentIntegratedTraditionalandWes~rnMedicineofOncology,TangduHospital,TheFourthMilitaryMedicalUniversity,Shaanxi皿a710038,China;StudentTeams,TheFourthMilitaryMedicalUniversity,ShaanxiXi"an710032,China;65547MilitaryHospital,LiaoningHaicheng114200,China;DepartmentRadiation
3、Medicine,TheFourthMilitaryMedicalUniversity,ShaanxiXi"an710032,China;DepartmentddChineseMedicine,ChinesePLAGeneralHospital,Beijing100853,China.【Abstract】Objective:ToinvestigatetheeffectofB—ElelnenecombinedwithDDPontheproliferationandapop—tosisofSiHacellMethods:Differentconcentrations
4、ofB—Elelneneinjection(25,50,100,150,200b~g/m1)andDDP(15,3,6,9,l2ln1)weresoloappliedtoSiHacelsCelproliferationwasnleasuredbyCCK一8(CelCountKit一8)Theappropriatedrugconcentration(3mlDDP,l25g/lnlp—Elelnene)wereselectedThen,p—E1一enlenecombinedwithDDPinjectionwasappliedtoSiHacelsCellprolife
5、rationwasnleasuredbyCCK一8Cellapop—tosiswasdetectedbyfloweytolnetryResults:CdlproliferationwasdramaticalyinhibitedbyB—Elelneneinjection(25,50,100,150,200b~g/ln1)andDDP(15,3,6,9,l2g/ln1)at24h,48h(P<005)inaddition,cellprolifera—tionwasdramaticallyinhibitedbyinjectionB—Elelnenecombinedwi
6、thDDPCombinationtherapy,apoptosisrateandinhibitionrateofproliferationinSiHacellstreatedbyB—ElenlenecombinedwithDDPwerehigherthanthel~lOnO—therapy(P<0f)1)Conclusion:DDP,B—Elel~leneandDDPcombinedwithelenlenecaninhibittheproliferation,acceleratetheapoptosisinSiHacellsDDPcombinedwithB—El
7、elnenehavesynergisticeffectontheapoptosisinSi—Hacalls【Keywords】Eleinene;DDP;SiHacdl;proliferation;apoptosisModernOncology2014,22(02):0265—0268【摘要】目的:探討B(tài)一欖香烯聯(lián)合順鉑對宮頸癌SiHa細胞生長的影響。方法:體外培養(yǎng)宮頸癌SiHa細胞,分別將p一欖香烯(濃度梯度為25、50、100、150、200m1),順鉑(濃度梯度為15?369、l2ln1),單獨作用于宮頸癌SiHa細胞,加藥24h、48h后用CCK一8法檢測
8、細胞增殖情況。用流式細胞