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《β-欖香烯聯(lián)合順鉑對(duì)乳腺癌 MCF -7細(xì)胞增殖和凋亡的影響-論文.pdf》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、匡堂生月第22卷第5期MODERNONCOLOGY.Mav.2014.VOL.22.NO.05·1005·l3一欖香烯聯(lián)合順鉑對(duì)乳腺癌MCF一7細(xì)胞增殖和凋亡的影響鄭瑾,馬力天,任秦有,楊建棟,劉毅,張毅,范玉貞,張偉,張哲偉,瞿湘梅,楊明會(huì)Efectofp—ElemenecombinedwithDDPoftheproliferationandapoptosisofMCF——7celIZhengJin,MaLitian,RenQinyu,YangJiandong,LiuYi,ZhangYi,F(xiàn)anYuzhen,Zhang
2、Wei,ZhangZhewei,QuXiangmei,YangMinghuiDepartmentofTraditionalChineseMedicine,ChinesePGeneralHospital,Beijing100853,China;DepartmentIntegratedTraditionalandWesternMedicineofOncology,TangduHospital,eFourthMilitaryMedicalUniversity,ShaanxiXihn710038,China;StudentTea
3、ms,TheFourthMilitaryMedicalUniversity,Shaanxian710032,China;465547MilitaryHospital,LiaoningHaicheng114200,China.【Abstract】Objective:ToinvestigatetheefectofB—ElemenecombinedwithDDPontheproliferationandapop.tosisofMCF一7cel1.Methods:DifferentconcentrationsofB—Elemen
4、einjection(25,50,100,150,2001zg/m1)wereappliedtoMCF一7cells.CellproliferationwasmeasuredbyMTr.Cellapoptosisandcell—cyclewasdetectedbyflowcytometry.Theappropriatedrugconcentration(3tLg/mlDDP,125tLg/mlB—Elemene)wereselected.Then8一E1-emenecombinedwithDDPinectionwasap
5、pliedtoMTrcells.CellproliferationwasmeasuredbyMTF.Cellapoptosisandcell—cyclewasdetectedbyfloweytometry.Results:CellproliferationwasdramaticallyinhibitedbyB—Elemeneinjection(25,50,100,150,2001xg/m1)at24h,48h(P<0.05).Inaddition,cellproliferationwasdramaticallyinhib
6、—itedbyinjectionB—ElemenecombinedwithDDP.Combinationtherapy,apoptosisrateandinhibitionrateofprolifera—tioninMCF一7cellstreatedbyp—E1emenecombinedwithDDPwashigherthanthemonotherapy(P<0.01).Cell—cyclewasarrestedinGo/G1phasebyelemenecomparedtouninfeetiouscellsinthesa
7、metimescale.Conclusion:p—ElemeneandDDPcombinedwithp—Elemenecaninhibittheproliferation,acceleratetheapoptosisinMCF一7cells.DDPcombinedwithB—ElemenehavesynergisticeffectontheapoptosisinMCF一7cells.【Keywords】B—Elemene;DDP;MCF一7cell;proliferation;apoptosis;cell—cycleMo
8、dernOncology2014.22(05):1005—1009【摘要】目的:探討B(tài)一欖香烯聯(lián)合順鉑對(duì)乳腺癌MCF一7細(xì)胞生長(zhǎng)的影響。方法:體外培養(yǎng)乳腺癌MCF一7細(xì)胞,將B一欖香烯(濃度梯度為25、50、100、150、2001xg/m1),單獨(dú)作用于乳腺癌MCF一7細(xì)胞,加藥24h、48h后用噻唑藍(lán)(MYr法)