資源描述：
《apelin對兩腎一夾高血壓大鼠離體主動脈環(huán)的舒張作用》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、Apelin對兩腎一夾高血壓大鼠離體主動脈環(huán)的舒張作用作者：莊紅，金戈，陳磊，楊鵬麟【摘要】目的:探討小分子活性肽Apelin-13對兩腎一夾（2K1C）高血壓大鼠離體主動脈環(huán)的舒張作用及意義。方法:①清潔級雄性SD大鼠27只，隨機分為正常對照組和2K1C高血壓模型組。測定平均頸動脈壓(mCAP)、左心重與體重比值(LVM/BW)以及右腎重/左腎重(RK/LK)，以評價2K1C模型。②采用累計加藥法，觀察Apelin-13分別對用去甲腎上腺素（NE）預(yù)收縮的對照組大鼠和2K1C高血壓組大鼠離體胸主動脈環(huán)張力的作用。結(jié)果:①模型組大鼠mCAP、LVM/BW和RK/LK分別較
2、對照組高50.38％(P<0.01)、32.16%(P<0.01)和51.02%(P<0.01)。②Apelin-13在（10-11～10-7）mol·L-1濃度范圍內(nèi)對內(nèi)皮完整的兩組大鼠胸主動脈環(huán)具有濃度依賴性的舒張作用，且模型組舒張率高于對照組(P<0.05)，而模型組與對照組EC50相比差異無顯著性(P>0.05)。結(jié)論:Apelin-13具有顯著的舒張主動脈的作用，并且對高血壓大鼠血管環(huán)的舒張作用更強，提示Apelin-13在血管的保護作用中扮演了重要角色?！娟P(guān)鍵詞】Apelin；主動脈；舒張作用；兩腎一夾；高血壓；腎性13Abstr
3、act:Objective:ToinvestigatetheeffectofapelinonvasodilatationofisolatedthoracicaortaringsinratsoftwoKindeyone-cliprenalhypertensionanditssignificance.Methods:①Twenty-sevenmaleSprague-Dawley（SD）ratswithweightof150～180gwererandomlydividedintotwogroups，controlrats(n=12)wasestimatedandmodelrat
4、softwoKindeyone-clip(2K1C)renalhypertension(n=15).Then，themodelof2K1Crenalhypertensionbymonitoringthemeancarotidarterialpressure(mCAP)，measuringtheratioofleftventricularmass(leftventricleplusseptum)tobodyweight(LVM/BW)，andrightkidneytotheleft(RK/LK).②Isolatedaortaringswereperfusedandwereg
5、iventherestingtension.Then，theeffectsofaccumulatedApelin-13onaortaringswithendotheliuminpre-constrictedwithnorepinephrine(NE)wereobserved.Results:①ThemCAPandLVM/BWof2K1Cgroupwere50.38%and32.16%higherthanthoseofthecontrolgroup，respectively(P<0.01，P<0.01).AndtheratioofRK/LKinthe2K1Cgr
6、oupwas51.02%higherthanthatinthecontrolgroup(P<0.01).②Apelin-13wasapotentvasodilatatoronratthoracicaorta.Apelin-13at10-11～10-7mol·L-1causeddose-depenentvasodilatationinendotheliumintactringspre-constrictedwithNE.Responseof13arteriesfromrenalhypertensionratswasincreased(P<0.05).Butthe
7、valuesofEC50werenotsignificantlydifferent(P>0.05).Conclusion:Theseresultsindicatethatapelin-13relaxesthethoracicaortainthetwogroupsandtheresponseofvasodilatationisenhancedintheratthoracicaortasfromthe2K1Chypertensiongroup.Itmaybeanimportantprotectingfactorandplay
