il1β511位點和tnfα308位點基因多態(tài)性與陜西漢族非賁門胃癌的關(guān)系

《il1β511位點和tnfα308位點基因多態(tài)性與陜西漢族非賁門胃癌的關(guān)系》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。

1、IL1β511位點和TNFα308位點基因多態(tài)性與陜西漢族非賁門胃癌的關(guān)系作者：賈皚，龔均，厲英超，萇新明，郝志明，董蕾【摘要】目的探討促炎癥因子白細(xì)胞介素(IL)1β基因啟動子區(qū)511位點及腫瘤壞死因子α(TNFα)308位點基因多態(tài)性與陜西漢族非賁門胃癌易感性的關(guān)系。方法用聚合酶鏈反應(yīng)和限制性片段長度多態(tài)性(PCRRFLP)的方法對陜西漢族106例非賁門胃癌及108例非潰瘍性消化不良患者(對照組)進(jìn)行IL1β啟動子的511位點和TNFα的308位點多態(tài)性分析。結(jié)果IL1β基因啟動子511位點基因型CC、CT、TT頻率在胃癌組和對照組分別為12.3%、54.7

2、%、33%和16.7%、50.9%、32.4%，無顯著性差異(P>0.05)。等位基因C、T頻率在胃癌組和對照組分別為39.6%、60.4%和42.1%、57.9%，其基因型頻率和等位基因頻率在胃癌組和對照組比較均無顯著性差異(P>0.05)。TNFα的308位點的基因型GG、GA、AA頻率在胃癌組和對照組分別為90.6%、1.9%、7.5%和84.3%、12.9%、2.8%，等位基因G、A頻率在胃癌組和對照組分別為91.5%、8.5%和85.6%、14.4%，其基因型頻率和等位基因頻率在胃癌組和對照組比較均無顯著性差異(P>0.05)。結(jié)論IL1β啟動子511位點基

3、因多態(tài)性及TNFα308基因多態(tài)性與陜西漢族非賁門胃癌之間未發(fā)現(xiàn)相關(guān)關(guān)系。14【關(guān)鍵詞】白細(xì)胞介素1；腫瘤壞死因子α；基因多態(tài)性；胃癌；漢族ABSTRACT:ObjectiveToinvestigatethecorrelationofthepolymorphismsoftheIL1βpromoterregion511C/Tandtumornecrosisfactorα(TNFα)308G/AgenewiththesusceptibilityofnoncardiacgastriccancerofShaanxiChinesepopulation.MethodsThepolymor

4、phismsintheIL1βpromoterregionandTNFαweredeterminedbyPCRRFLPinaHannationalityofShaanxiChinesepopulation.Totally106casesofnoncardiacgastriccancerand108casesofnonulcerdyspepsiaascontrolsweresubjectedtoIL1βandTNFαgeneanalysis.ResultsIL1βgenotypefrequenciesofCC,CTandTTwere12.3%,54.7%and33%ingastr

5、iccancergroup;16.7%,50.9%,32.4%incontrolgrouprespectively.AllelefrequenciesofCandTwere39.6%,60.4%ingastriccancergroup;42.1%and57.9%incontrolgrouprespectively.TNFα308genotypefrequenciesofGG,GAandAAwere90.6%,1.9%and7.5%ingastriccancergroup;84.3%,12.9%,2.8%incontrolgrouprespectively.Allelefrequencies

6、ofGandAwere91.5%and8.5%ingastriccancergroup;85.6%and14.4%incontrolgrouprespectively.Therewerenosignificantdifferencesintheallelefrequencyandgenotypeof14IL1β511C/TandTNFα308(P>0.05).ConclusionNeitherthe511C/TpolymorphismintheIL1βpromoterregionnorTNFα308genepolymorphismwasassociatedwithsusc

7、eptibilityofnoncardiacgastriccancerinaHannationalityofShaanxiChinesepopulation.KEYWOEDS:interleukin1β;tumornecrosisfactorα;genepolymorphism;gastriccancer;Chinesepopulation　胃癌是人類最常見的惡性腫瘤之一，陜西是胃癌的高發(fā)區(qū)