資源描述：
《il-17基因多態(tài)性與漢族人群胃癌相關(guān)性研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、授予單位代碼10089學(xué)號(hào)或申請(qǐng)?zhí)?3524HebeiMedicalUniversity碩士學(xué)位論文在職科學(xué)學(xué)位IL-17基因多態(tài)性與漢族人群胃癌相關(guān)性研究作者姓名：高偉導(dǎo)師：楊麗娟副教授專業(yè)：免疫學(xué)二級(jí)學(xué)院：河北醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院2015年03月河北醫(yī)科大學(xué)學(xué)位論文使用授權(quán)及知識(shí)產(chǎn)權(quán)歸屬承諾本學(xué)位論文在導(dǎo)師(或指導(dǎo)小組)的指導(dǎo)下,由本人獨(dú)立完成。本學(xué)位論文研究所獲得的研究成果，其知識(shí)產(chǎn)權(quán)歸河北醫(yī)科大學(xué)所有。河北醫(yī)科大學(xué)有權(quán)對(duì)本學(xué)位論文進(jìn)行交流、公開和使用。凡發(fā)表與學(xué)位論文主要內(nèi)容相關(guān)的論文,第一署名為單位河北醫(yī)科大學(xué)，試驗(yàn)材料、原始
2、數(shù)據(jù)、申報(bào)的專利等知識(shí)產(chǎn)權(quán)均歸河北醫(yī)科大學(xué)所有。否則,承擔(dān)相應(yīng)法律責(zé)任。研究生簽名‘導(dǎo)師簽章級(jí)學(xué)院領(lǐng)導(dǎo)蓋章：贈(zèng)年Jr月巧曰河北醫(yī)科大學(xué)研究生學(xué)位論文獨(dú)創(chuàng)性聲明本論文是在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果，除了文中特別加以標(biāo)注和致謝等內(nèi)容外，文中不包含其他人已經(jīng)發(fā)表或撰寫的研究成果，指導(dǎo)教師對(duì)此進(jìn)行了審定。本論文由本人獨(dú)立撰寫，文責(zé)自負(fù)。研究生簽名導(dǎo)師簽章:年上月巧曰目錄中文摘要·············································································
3、1英文摘要·············································································4研究論文IL-17基因多態(tài)性與漢族人群胃癌相關(guān)性研究前言·············································································8材料與方法····································································9結(jié)果·····
4、········································································17附圖·············································································21附表·············································································27討論································
5、·············································31結(jié)論·············································································34參考文獻(xiàn)·······································································35綜述IL-17基因多態(tài)性與胃癌的關(guān)系········································39致謝···
6、················································································56個(gè)人簡歷·············································································57中文摘要IL-17基因多態(tài)性與漢族人群胃癌相關(guān)性研究摘要目的：通過采用PCR-RFLP技術(shù)及直接測(cè)序法對(duì)漢族胃癌人群IL-17基因已知的SNP位點(diǎn)rs2275913,rs3748067,rs4711998和rs
7、763780進(jìn)行研究，比較各SNP位點(diǎn)等位基因在胃癌及正常對(duì)照人群中的分布，揭示IL-17基因的SNP位點(diǎn)與胃癌之間的相互關(guān)系。方法：本課題包括2013-2014年從河北省人民醫(yī)院收集的386例經(jīng)過內(nèi)鏡檢查病理組織學(xué)確診的胃癌患者及374例同期健康體檢者，兩組研究人群在年齡、性別構(gòu)成上均相匹配。排除自身免疫及炎癥性疾病，如系統(tǒng)性紅斑狼瘡、糖尿病、類風(fēng)濕關(guān)節(jié)炎、炎癥性腸病等，排除有胃癌家族史者，所有研究對(duì)象經(jīng)血、尿、便常規(guī)和心電圖檢查均未發(fā)現(xiàn)異常。取4mlEDTA-K2抗凝血，提取基因組DNA,采用生物學(xué)軟件Primer5.0設(shè)計(jì)引物，
8、并與GenBank進(jìn)行比對(duì)。采用PCR-RFLP方法擴(kuò)增目的基因片段，進(jìn)行多態(tài)性分析。統(tǒng)計(jì)數(shù)據(jù)在SPSS19.0軟件包中完成，各統(tǒng)計(jì)指標(biāo)均以均數(shù)±標(biāo)準(zhǔn)差（mean±SD）表示。用基因計(jì)數(shù)法計(jì)算各基因型頻率和等位2基因頻率
