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1、授予單位代碼10089學號或申請?zhí)?3524HebeiMedicalUniversity碩士學位論文在職科學學位IL-17基因多態(tài)性與漢族人群胃癌相關性研究作者姓名：高偉導師：楊麗娟副教授專業(yè)：免疫學二級學院：河北醫(yī)科大學基礎醫(yī)學院2015年03月河北醫(yī)科大學學位論文使用授權(quán)及知識產(chǎn)權(quán)歸屬承諾本學位論文在導師(或指導小組)的指導下,由本人獨立完成。本學位論文研究所獲得的研究成果，其知識產(chǎn)權(quán)歸河北醫(yī)科大學所有。河北醫(yī)科大學有權(quán)對本學位論文進行交流、公開和使用。凡發(fā)表與學位論文主要內(nèi)容相關的論文,第一署名為單位河北醫(yī)科大學，試驗材料、原始
2、數(shù)據(jù)、申報的專利等知識產(chǎn)權(quán)均歸河北醫(yī)科大學所有。否則,承擔相應法律責任。研究生簽名‘導師簽章級學院領導蓋章：贈年Jr月巧曰河北醫(yī)科大學研究生學位論文獨創(chuàng)性聲明本論文是在導師指導下進行的研究工作及取得的研究成果，除了文中特別加以標注和致謝等內(nèi)容外，文中不包含其他人已經(jīng)發(fā)表或撰寫的研究成果，指導教師對此進行了審定。本論文由本人獨立撰寫，文責自負。研究生簽名導師簽章:年上月巧曰目錄中文摘要·············································································
3、1英文摘要·············································································4研究論文IL-17基因多態(tài)性與漢族人群胃癌相關性研究前言·············································································8材料與方法····································································9結(jié)果·····
4、········································································17附圖·············································································21附表·············································································27討論································
5、·············································31結(jié)論·············································································34參考文獻·······································································35綜述IL-17基因多態(tài)性與胃癌的關系········································39致謝···
6、················································································56個人簡歷·············································································57中文摘要IL-17基因多態(tài)性與漢族人群胃癌相關性研究摘要目的：通過采用PCR-RFLP技術及直接測序法對漢族胃癌人群IL-17基因已知的SNP位點rs2275913,rs3748067,rs4711998和rs
7、763780進行研究，比較各SNP位點等位基因在胃癌及正常對照人群中的分布，揭示IL-17基因的SNP位點與胃癌之間的相互關系。方法：本課題包括2013-2014年從河北省人民醫(yī)院收集的386例經(jīng)過內(nèi)鏡檢查病理組織學確診的胃癌患者及374例同期健康體檢者，兩組研究人群在年齡、性別構(gòu)成上均相匹配。排除自身免疫及炎癥性疾病，如系統(tǒng)性紅斑狼瘡、糖尿病、類風濕關節(jié)炎、炎癥性腸病等，排除有胃癌家族史者，所有研究對象經(jīng)血、尿、便常規(guī)和心電圖檢查均未發(fā)現(xiàn)異常。取4mlEDTA-K2抗凝血，提取基因組DNA,采用生物學軟件Primer5.0設計引物，
8、并與GenBank進行比對。采用PCR-RFLP方法擴增目的基因片段，進行多態(tài)性分析。統(tǒng)計數(shù)據(jù)在SPSS19.0軟件包中完成，各統(tǒng)計指標均以均數(shù)±標準差（mean±SD）表示。用基因計數(shù)法計算各基因型頻率和等位2基因頻率
