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《survivin 反義寡核苷酸誘導(dǎo)胃癌原代細(xì)胞凋亡的實(shí)驗(yàn)研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、survivin反義寡核苷酸誘導(dǎo)胃癌原代細(xì)胞凋亡的實(shí)驗(yàn)研究【摘要】目的探討survivin反義寡核苷酸對(duì)人胃癌原代細(xì)胞凋亡的影響，為胃癌的靶向治療提供實(shí)驗(yàn)依據(jù)。方法利用人胃癌實(shí)體瘤新鮮標(biāo)本培養(yǎng)胃癌原代細(xì)胞，胃癌細(xì)胞分為空白對(duì)照組、脂質(zhì)體組、正義寡核苷酸組及反義寡核苷酸轉(zhuǎn)染組。用脂質(zhì)體介導(dǎo)survivin反義寡核苷酸轉(zhuǎn)染胃癌原代細(xì)胞，48h后，觀察腫瘤細(xì)胞的形態(tài)變化，Westernblot檢測(cè)survivin蛋白的表達(dá)，流式細(xì)胞術(shù)檢測(cè)細(xì)胞的凋亡。結(jié)果反義寡核苷酸轉(zhuǎn)染組腫瘤細(xì)胞體積變小，細(xì)胞碎裂，出現(xiàn)凋亡小體；survi
2、vin蛋白量下降；流式細(xì)胞術(shù)檢測(cè)到腫瘤細(xì)胞的凋亡率增高。與其他各組相比，其差異有統(tǒng)計(jì)學(xué)意義（P<0.05），而空白對(duì)照組、脂質(zhì)體組、正義寡核苷酸組之間的差異無(wú)統(tǒng)計(jì)學(xué)意義（P>0.05）。結(jié)論survivin反義寡核苷酸在脂質(zhì)體介導(dǎo)下轉(zhuǎn)染人胃癌原代細(xì)胞，可以誘導(dǎo)腫瘤細(xì)胞發(fā)生凋亡，抑制細(xì)胞生長(zhǎng)，反義survivin可能成為一個(gè)促進(jìn)胃癌細(xì)胞凋亡的手段?！娟P(guān)鍵詞】survivin；反義寡核苷酸；凋亡；原代細(xì)胞；胃癌Researchoneffectsofsurvivinantisenseoligonucleotid
3、einducingapoptosisofprimarycellofhumangastriccarcinoma［Abstract］ObjectiveToinvestigatetheeffectsofsurvivin12antisenseoligonucleotideoninducingapoptosisofprimarycellofgastriccarcinomaandprovidetheevidenceinexperimentforthetargetingtherapyofgastriccarcinoma.Metho
4、dsTheprimarycellswereculturedwiththefreshtissuefromhumangastriccarcinoma.Theculturedprimarycellswerecollectedanddividedintofourgroups:shamcontrolgroup，liposomescontrolgroup，SODNcontrolgroupandASODNexperimentgroup.Aftertransfectingcellsfor48hoursusingsynthesized
5、survivinASODNbyliposome，culturedcellwereharvestedtocarryoutnexttests:primarycellmorphologicalchangeswereobservedbytheinvertedmicroscope，survivinproteinexpressionwasdetectedbyWesternblot，cellapoptosiswasexaminedbyflowcytometry.ResultsPrimarycellsweretransfecteda
6、fter48hours，abnormalmorphologicalchangeofprimarycellwasobservedinASODNtransfectedgroup.TheexpressionofsurvivinproteininASODNgroupwasdecreasedascomparedwithothercontrolgroupsbyWesternblotdetection.TheflowcytometryshowedtheARofASODNgroupwassignificantlyhigherthan
7、thatofothergroups.TherewerestatisticaldifferencesbetweenASODNgroupandtheothergroups（P<0.05），andnodifferencesamongshamgroup，lipsomegroupandlip-SODNgroup（P>0.05）.ConclusionSurvivinantisenseoligonucleotidecaninducetheapoptosisand12inhibitthegrowthoftheprimar
8、ycellofgastriccarcinoma.SurvivinASDONmightbeamethodtopromotetheapoptosisofthecellofgastriccarcinoma.［Keywords］survivin;antisenseoligonucleotide;apoptosis;primarycell;gastric
