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《survivin反義寡核苷酸激活caspase-3誘導(dǎo)sgc7901胃癌細(xì)胞凋亡的實(shí)驗(yàn)研究》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、survivin反義寡核苷酸激活caspase-3誘導(dǎo)SGC7901胃癌細(xì)胞凋亡的實(shí)驗(yàn)研究【摘要】目的研究轉(zhuǎn)染生存素(survivin)反義寡核苷酸誘導(dǎo)SGC7901胃癌細(xì)胞凋亡及其可能的作用機(jī)制。方法實(shí)驗(yàn)分為空白對照組(Control組)、脂質(zhì)體組（Lip組）、正義寡核苷酸組（Sodn組）及反義寡核苷酸組(Asodn組)。人工合成正、反義寡核苷酸，經(jīng)脂質(zhì)體將survivin正、反義寡核苷酸轉(zhuǎn)染入胃癌細(xì)胞48h后，MTT檢測細(xì)胞增殖抑制率，Westernblot檢測caspase-3蛋白表達(dá)改變，Hoechst染色觀察細(xì)胞凋亡形態(tài)
2、改變及流式細(xì)胞儀檢測凋亡率。結(jié)果Hoechst染色熒光顯微鏡下Control組、Lip組及Sodn組細(xì)胞核呈正常的藍(lán)色，而Asodn組細(xì)胞核染色增強(qiáng)、核質(zhì)濃縮、核碎裂，呈凋亡改變；Asodn組細(xì)胞增殖抑制率、細(xì)胞凋亡率、caspase-3蛋白表達(dá)均增高，與Control組、Lip組及Sodn組相比差異均有統(tǒng)計(jì)學(xué)意義(P<0.05)。結(jié)論survivin反義寡核苷酸可誘導(dǎo)胃癌細(xì)胞凋亡，其作用機(jī)制可能是通過激活caspase-3表達(dá)，啟動凋亡信號傳導(dǎo)?！娟P(guān)鍵詞】生存素；反義寡核苷酸；caspase-3；胃癌細(xì)胞；凋亡Abstra
3、ct:ObjectiveToinvestigatetheeffectoftransfectedsurvivinantisenseoligonucleotideoninducingapoptosisingastriccancercellsandthepossiblecausativemechanism.MethodsTheexperimentincludedfourgroups:control,liposome9(Lip),senseoligonucleotide(Sodn)andantisenseoligonucleotide(A
4、sodn).Artificiallysynthesizedsurvivinantisenseoligonucleotidewastransfectedfor48hoursintogastriccancercellsusingliposome.Aftercultivation,thecellproliferationinhibitionratewasevaluatedbyMTTassay,theexpressionofcaspase-3proteinwasdeterminedwithWesternblot,andthemorphol
5、ogicalchangesofapoptosiswereobservedbyHoechststaining,andtheapoptosisratewastestedwithAnnexinv-FITCassayofflowcytometricanalysis.ResultsFluorescencemicroscopicobservationsrevealedthatHoechststaininginthecontrol,LipandSodngroupshadnucleuspresentedasnormalblue,whiletheA
6、sodngrouphadsuchchangesasreinforcednucleusstaining,karyoplasmconcentration,karyorrhexisandapoptoticmodifications.Asodngrouphadasignificantincreaseinthecellproliferationinhibitionrate,cellapoptosisrateandexpressionofcaspase-3protein,comparedwiththecontrol,LipandSodngro
7、ups.Thedifferenceshadstatisticalsignificance(P<0.05).ConclusionSurvivinantisenseoligonucleotidecaninduceapoptosisofgastriccancercells,Itspossiblemechanismmightbethattheapoptosissignaltransductionisinitiatedbytheactivationofcaspase-3expression.9Keywords:survivin;ant
8、isenseoligonucleotide;caspase-3;gastriccancercell;apoptosis英文已換生存素(survivin)是近期發(fā)現(xiàn)的一種凋亡抑制蛋白家族成員，也是迄今發(fā)現(xiàn)的作用最強(qiáng)的凋亡抑制因子，survivin的過
