冬蟲(chóng)夏草對(duì)糖尿病大鼠纖溶系統(tǒng)的影響

《冬蟲(chóng)夏草對(duì)糖尿病大鼠纖溶系統(tǒng)的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。

1、冬蟲(chóng)夏草對(duì)糖尿病大鼠纖溶系統(tǒng)的影響作者：陳葉傅余芹方華偉韓亞莉杜月娟【摘要】　　目的觀察冬蟲(chóng)夏草(CS)對(duì)糖尿病腎病(DN)大鼠纖溶系統(tǒng)功能的影響。方法44只大鼠隨機(jī)分為正常對(duì)照組、模型組(給予鏈脲佐菌素55mg/kg體重腹腔注射)，CS早期干預(yù)組及冬蟲(chóng)夏草晚期干預(yù)組，12w后測(cè)體重、腎重、血糖、血肌酐(Scr)、24h尿蛋白等指標(biāo)，光鏡觀察腎臟病理變化，免疫組化法分別檢測(cè)組織型纖溶酶原激活物(tPA)/纖溶酶原激活物抑制劑表達(dá)(PAI)。結(jié)果模型組腎重、血糖、Scr、24h尿蛋白均明顯高于正常對(duì)照組(P＜0.01

2、)，PAI表達(dá)明顯高于正常對(duì)照組，體重、tPA表達(dá)明顯低于正常對(duì)照組；兩干預(yù)組腎重、Scr、血尿素氮(BUN)、24h尿蛋白、tPA表達(dá)均高于模型組(P＜0.05)，低于正常對(duì)照組(P＜0.01)，體重、PAI表達(dá)低于模型組，高于正常組，血糖較模型組無(wú)明顯變化；冬蟲(chóng)夏草早期干預(yù)組效果優(yōu)于晚期干預(yù)組(P＜0.05)。結(jié)論DN時(shí)存在tPA/PAI失衡，冬蟲(chóng)夏草治療DN的機(jī)制之一為矯正tPA/PAI失衡?！娟P(guān)鍵詞】冬蟲(chóng)夏草；糖尿病腎??；纖溶酶原激活劑；纖溶酶原激活劑抑制劑【Abstract】ObjectiveToi

3、nvestigatetheeffectsofcordycepssinensis(CS)onfibrinolyticsystemofdiabeticrats.11Methods44ratsweredividedinto4groups:control(n=10),model(n=12,intraperitonealinjectionwith55mg/kgSTZ),earlyandlateCStreatedgroups(n=11).1ratwasfailedtomademodel,1ratdiedinmodelgroupa

4、nd1ratdiedinearlyCStreatedgroup.12weekslater,bodyweight,renalweight,serumglucose,serumcreatinine,24hoururinaryproteinexcretionwereexamined.Therenalpathologicalchangeswereobservedbylightmicroscopeandthelocalexpressionsofrenalplasminogenactivatorinhibitor1(PAI

5、1)andtissuetypeplasminogenactivator(tPA)weremeasuredbyimmunohistochenmicaltechnique.ResultsInmodelgroup,renalweight,serumglucose,serumcreatinine,24hoururinaryproteinexcretionandPAIwereincreasedsignificantly(P＜0.01),whilebodyweightandtPAweredecreasedcompared

6、withcontrolgroup(P＜0.01).Therenalweight,serumcreatinine,24hoururinaryproteinexcretion,tPAwereincreasedinearlyandlateCStreatedgroupscomparedwiththoseofmodelgroup(P＜0.05),andtheaboveindexesweredecreasedcomparedwiththoseofcontrolgroup(P＜0.01).Bodyweight,PAIwered

7、ecreasedinearlyandlatetreatedgroupscomparedwiththoseofmodelgroupwhilewereincreasedcomparedwiththoseofcontrolgroup(P＜0.05).Serumglucoseininearlyandlatetreatedgroupshadnoobviousdifferencecomparedwith11modelgroup.TheeffectinearlyCStreatedgroupwasbetterthanthatofla

8、teCStreatedgroup(P＜0.05).ConclusionsThereisdisbalanceindiabeticnephropathyandtherectificationofthetPA/PAIdisbalanceisoneofmechanismsofCStreatingdiabeticnephropathy.【Keyword