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1、www.CRTER.org莊，等.黃芩Hypoxicpreconditioningeffectsontheabilityofbonemarrowmesenchymalstemcellstoleranttoischemiaandhypoxia*XuQiao-yan1,XiaLin2,WangJi-chang31DepartmentofRespiratoryMedicine,thePeople’sHospitalofLaizhou,Yantai261400,ShandongProvince,China2Departmento
2、fPlasticSurgery,QiluHospitalofShandongUniversity,Jinan250012,ShandongProvince,China3DepartmentofPlasticSurgery,theSecondHospitalofShandongUniversity,Jinan250033,ShandongProvince,ChinaXuQiao-yan,Attendingphysician,DepartmentofRespiratoryMedicine,thePeople’sHospita
3、lofLaizhou,Yantai261400,ShandongProvince,Chinaqiaoyanx@yahoo.cnCorrespondingauthor:WangJi-chang,M.D.,Attendingphysician,DepartmentofPlasticSurgery,theSecondHospitalofShandongUniversity,Jinan250033,ShandongProvince,Chinawangjichangxu@163.comAccepted:2013-09-18(201
4、303132/WJ)AbstractBACKGROUND:Accordingtothepreviousstudies,itisknownthatbonemarrowmesenchymalstemcellsliveinthephysiologicalenvironmentofloweroxygenconcentrationcomparedwiththecommoncultureconcentration(20%-21%).Hypoxiacanpromotebonemarrowmesenchymalstemcellsprol
5、iferationandmaintainthecharacteristicsofdifferentiationpotential.OBJECTIVE:Tounderstandthebiologicalcharacteristicsofbonemarrowmesenchymalstemcellsafterhypoxicpreconditioning,andsearchforinvitrobasisforbonemarrowmesenchymalstemcellstherapyinvivowithhypoxicprecond
6、itioningstrategy.METHODS:Afterpassageandinoculation,bonemarrowmesenchymalstemcellswereculturedinthehypoxicincubator(1%O2,5%CO2).Bonemarrowmesenchymalstemcellsculturedinthenormoxicincubator(20%O2,5%CO2)servedascontrols.RESULTSANDCONCLUSION:Comparedwithbonemarrowme
7、senchymalstemcellsculturedundernormoxia(20%O2),bonemarrowmesenchymalstemcellsculturedunderhypoxia(1%O2)begantodisplayexponentialgrowthphaseafteralongerincubationperiod.Hypoxia(1%O2)wasnotthelethalconditionforbonemarrowmesenchymalstemcells.Cellsurvivalratesofbothg
8、roupsreducedovertime,whilethoseofbonemarrowmesenchymalstemcellsculturedundernormoxiareducedfaster(P<0.05).Surfacemarkersexpressionofbonemarrowmesenchymalstemce
