資源描述：
《二氮嗪預(yù)處理對(duì)肝硬化大鼠肝臟的保護(hù)作用.doc》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、二氮嗪預(yù)處理對(duì)肝硬化大鼠肝臟的保護(hù)作用作者：史衛(wèi)海，孫亞偉，徐學(xué)忠，戎亞雄，李文美【摘要】目的探討采用線粒體ATP敏感性鉀通道(mitoKATP通道)選擇性開放劑二氮嗪(DE)進(jìn)行預(yù)處理能否模擬缺血預(yù)處理(IP)對(duì)硬化肝臟缺血再灌注損傷的保護(hù)作用及其可能的作用機(jī)制。方法雄性肝硬化SD大鼠隨機(jī)分為5組(每組8只)。IP組以肝缺血5min作預(yù)處理；DE組以靜脈注射DE作為預(yù)處理；DE+5-HD組是在DE組基礎(chǔ)上再予靜注mitoKATP通道選擇性阻滯劑5-hydroxydecanoate(5-HD)進(jìn)行預(yù)處理;對(duì)照
2、組(C組)以靜注等量生理鹽水作為預(yù)處理;上述4組均在預(yù)處理后行肝缺血45min再灌注60min;缺血方式均為70%肝臟熱缺血。假手術(shù)組（S組）僅行開腹，不作任何其他處理。完成預(yù)定實(shí)驗(yàn)操作后分別取血用于血清谷丙轉(zhuǎn)氨酶(ALT)與乳酸脫氫酶(LDH)檢測(cè)，切取肝組織用于測(cè)定ATP酶活力、濕重/干重(W/D)的測(cè)定及觀察顯微、超微結(jié)構(gòu)變化。結(jié)果C組ALT，LDH，ATP酶及W/D的水平明顯高于S組(P<0.01)，肝臟的顯微及超微結(jié)構(gòu)損傷明顯；IP組與DE組的各項(xiàng)肝組織損傷指標(biāo)均明顯好于C組，ATP酶活性低于
3、C組(P<0.05及P<0.01)；而DE+5-HD組的肝損傷指標(biāo)均差于DE組，ATP酶活性高于DE組(P<0.05及P<0.01)。結(jié)論使用DE進(jìn)行藥物預(yù)處理能夠模擬出IP效應(yīng)，對(duì)肝硬化大鼠肝臟I/R損傷具有保護(hù)作用，其作用可能與下調(diào)肝組織ATP酶活性，減少ATP大量分解，使肝組織ATP含量升高，改善肝能量代謝，增加能量?jī)?chǔ)備;改善肝組織微循環(huán)，減輕肝臟水腫有關(guān)?！娟P(guān)鍵詞】缺血預(yù)處理；缺血/再灌注；線粒體ATP敏感性鉀通道；肝硬化；二氮嗪；大鼠AbstractObjectiveToex
4、plorewhetherthepretreatmentwithdiazoxide(DE)—aselectiveopenerofmitochondrialATPsensitivepotassium(mitoKATP)channelscanmimictheprotectivefunctionofischemicpreconditioning(IP)onliverischemia/reperfusion(I/R)injuryinratswithcirrhosisandtheprobablemechanism.Met
5、hodsMaleSDratswithlivercirrhosiswererandomlydividedintofivegroups(n=8ineachgroup).Pretreatmentwithhepaticischemiafor5minwasregardedasIPgroup；pretreatmentwithDE5mg/kgintravenouslyinjectionwasregardedasDEgroup；pretreatmentwithintravenousinjectionofaselectivem
6、itoKATPchannelsinhibitor5-hydroxydecanoate(5-HD)baseduponDEgroupwasconsideredasDE+5-HDgroupandpretreatmentwithnormalsalineinequalvolumeasacontrolgroup（Cgroup）.Thefourgroupsmentioned-aboveweresubjectedtohepaticischemiafor45minafterpretreatment,then,50minutes
7、reperfusionwasperformedonthem.Allofratsweresubjectedto70%warmischemia.Theadditionalfifth6groupofratswasundergoneashamoperation(Sgroup)，inwhomonlyanesthesiaandlaparotomywereperformedwithoutanyothermeasures.Bloodandlivertissuewereobtainedtodeterminethebiochem
8、istrylevelsandobservepathologicalchanges.ResultsTheactivitiesofALT，LDH，ATPaseandthelevelsofW/DinCgroupwerehigherthanthoseinSgroup（P＜0.01）.Therewerealsoseverehistologicalandultra-structuraldamagesinCgro
