二氮嗪預(yù)處理對(duì)大鼠腦缺血再灌注損傷后血腦屏障的保護(hù)作用分析

《二氮嗪預(yù)處理對(duì)大鼠腦缺血再灌注損傷后血腦屏障的保護(hù)作用分析》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。

1、·英文論著摘要·Researchontheprotectiveeffectofdiazoxidepretreat--．mentontheblood．brainbarrierofratsaftercerebralischemia／reperfusioninjuryobjeetiveTostudytheeffectanditsmechanismofdiazoxideontheblood-brainbarrier(BBB)ofratsaftercerebralischemia／reperfusion(I／R1injury．MethodsSixtyWista

2、rratswererandomlydividedintoshamoperationgroup、I／Rgroupanddiazoxidepretreatmentgroupsoflow、middle、largedose(5、10、20mg／kg)，TheI／Rmodelsofratswereperformedtoundergomiddlecerebralarteryembolismbythread．BBBpermeabilitywasestimatedbyEvan’S—blue(EB)dyeing，Transmissionelectronmicrosco

3、pe(TEM)Wasscheduledtoobservethemodificationofinterendothelialtightjunction(TJ)ofcapillaries，theexpressionofClaudin-5andZo-1ineveryratbraintissueswasdetectedbyimmunityhistochemistrytechnique．Resuits1．Comparedwithshamoperationgroup，ThepermeabilityextentofEBweresignificantlyincrea

4、sedbyI／Rwhichwasdistinctlyattenuatedinmiddleandlargedoseofdiazoxidepretreatmentrats，whilenoobviouschangeswerefoundbetweenFRandlowdosegroups．2．TEMshowedthatTJofthebraintissueopenedafterURinjuryandnosignificantopeningofTJWasobservedinmiddleandlargedoseofdiazoxidepreconditioninggo

5、ups．33．Comparedwithshamoperationgroup，theexpressionofClaudin一5andZo一1inthebraintissueoftheI／Rgroupwereapparentlydecreased(P

6、noobviousdifferencesbetweenlowdosegroup(5mg／kg)andtheI／Rgroup(P>0．05)．ConclusionPreconditioningofischemia／reperfusioninjurywithdiazoxideprotectstheblood-brainbarrier，whichmayduetotheincreasedexpressionoftightjunction-associatedproteinsClaudin-5andZo-1．Keywordsblood-brainbarrier

7、；diazoxide；ischemia／reperfusioninjury；tightjunctionClaudin·-5；Zo·-14·英文縮略語·5·論文·二氮嗪預(yù)處理對(duì)大鼠腦缺血再灌注后血腦屏障損傷的保護(hù)作用研究吉釘吉莉青2001年7月美國國立神經(jīng)病學(xué)與卒中研究所(NationalInstitutesofNeurologicalDisordersandStroke，NINDS)提出了“神經(jīng)血管單元”(neurovascularunit，NVU)的概念。近期研究發(fā)現(xiàn)卒中可以看做是多種細(xì)胞共同參與的腦功能紊亂。NVU構(gòu)成了在卒中及其治療中細(xì)胞一細(xì)胞

8、間傳遞信號(hào)和細(xì)胞一基質(zhì)問相互影響的架構(gòu)，為卒中研究提供了的新方向和藥物干預(yù)的新靶點(diǎn)，其結(jié)構(gòu)基礎(chǔ)主要包括神經(jīng)元