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1、西南農(nóng)業(yè)學(xué)報(bào)2017年3O卷6期1340SouthwestChinaJournalofAgriculturalSciencesV0l_3ONo.6文章編號(hào):1001—4829(2017)6—1340—06DOI:10.16213/j.cnki.scjas.2017.6.018玉米莖腐、鞘腐、穗腐病中不同鐮孢菌間關(guān)系研究袁丁,何鵬飛,吳毅歆,郭力維,袁遠(yuǎn),何月秋。,4}(1.云南農(nóng)業(yè)大學(xué)農(nóng)業(yè)生物多樣性應(yīng)用技術(shù)國家工程研究中心,云南昆明650201;2.濱州職業(yè)學(xué)院,山東濱州256603;3.微生物菌種篩選與應(yīng)用國家地方聯(lián)合工程研究中心,云南昆明650217;4.云南農(nóng)
2、業(yè)大學(xué)農(nóng)學(xué)與生物技術(shù)學(xué)院,云南昆明650201)摘要:【目的l為了明確不同鐮孢菌對玉米莖腐病、鞘腐病和穗腐病的致病性?!痉椒ā坎捎猛寥澜泳⒋┐探泳玩咦討腋∫航泳姆椒??!窘Y(jié)果1證明層出鐮孢菌(Fusariumproliferatum)、輪枝鐮孢菌(verticillioides)可引起玉米莖腐病,禾谷鐮孢菌(柙一minearum)可引起穗腐病、鞘府病,而尖孢鐮孢菌(F.oxysporum)則通過復(fù)合侵染引起病害?!窘Y(jié)論】將各類鐮孢菌分別接種不同生育期的玉米植株,觀察株高、病斑大小、玉米發(fā)育程度和籽粒的形成情況,研究各鐮孢茵菌株對玉米植株的影響程度,分析各菌株的致
3、病力,最佳侵染時(shí)期及莖腐、鞘腐、穗腐之間的侵染關(guān)系,證明玉米莖腐、鞘腐、穗腐之間病原菌侵染部位沒有特異性,不同時(shí)期侵染引起的莖腐、鞘腐及穗腐發(fā)病程度不同。關(guān)鍵詞:玉米;鐮孢菌;致病性;關(guān)系中圖分類號(hào):s513文獻(xiàn)標(biāo)識(shí)碼:AAnalysisofRelationshipamongFusariumPathogensofStalk,EarandSheathRotsofMaizeYUANDing,HEPeng—fei,WUYi-xin,GUOLi.wei,YUANYuan,HEYue.qiu,(1.NationalEngineeringCenterforAppliedTech
4、niquesofAculturalBiodiversity,YunnanAg6culturalUniversity,YunnauKunming650201,China;2.BinzhouPolytechnic,ShandongBinzhou256603,China;3.NationalandLocalJointEngineeringResearchCenterforMicrobialScreeningandApplication,YunnanKunming650217,China;4.FacultyofAgronomyandBiotechnology,YunnanA
5、griculturalU—niversity,YunnanKuuming650201,China)Abstract:【Objective】Theobjectiveofthisstudyistounderstandthepathogenicityofstalk,sheathandearrotspathogens.【Method】Threekindsofmethodswhicharesoilinoculation,punctureinoculationandsporesuspensioninoculationwereused.【Result】Stalkrotsofmai
6、zewasmainlycausedbyFusariumproliferatumandF.verticillioides.a(chǎn)nddirectinoculationconfirmedthatF.graminearumcausedearandsheathrotsofmaizeandoxysporumcausedmanytypesofrotsbyaccompaniedinfection.【Conclusion】Byinoculationofthepathogenson—tomaizesheath,earandstalkatvariousperiods,plantheight
7、,lesionsize,grainfullnessofmaizewereobservedforanalyzingtheirpathoge—nicity,bestinfectionperiodandtherelationshipsamongthepathogensofstalk,sheathandearrots.ItisconcludedthatallofthefourFusar-iumpathogenscouldinfectstalk,sheathandearwithoutpartsspecializationthroughwoundalthoughtheirp