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1、維普資訊http://www.cqvip.com藥學(xué)學(xué)報(bào)ActaPharmaceuticaSinica2008，43(6)：591—595·591·?；撬釋?duì)大鼠心肌成纖維細(xì)胞增殖的影響任曠，王艷春，楊世杰(1．吉林醫(yī)藥學(xué)院基礎(chǔ)醫(yī)學(xué)院，吉林吉林132013；2．吉林大學(xué)白求恩醫(yī)學(xué)院，吉林長春130021)摘要：觀察?；撬?taurine，Tau)在血管緊張素II(angiotensinII，AngII)誘導(dǎo)新生大鼠心肌成纖維細(xì)胞(cardiacfibroblast，CFb)增殖過程中對(duì)一氧化氮(nitricoxide，NO)及蛋白激酶Calpha(p-PKCa)的影響，以探討牛磺酸抑制CF
2、b增殖的信號(hào)轉(zhuǎn)導(dǎo)途徑。胰酶消化法分離培養(yǎng)新生大鼠CFb，用AngII誘導(dǎo)促進(jìn)其增殖，采用MTT法檢測細(xì)胞增殖；ELISA法測定I型膠原、III型膠原的含量；流式細(xì)胞儀檢測細(xì)胞周期；硝酸還原酶法檢測細(xì)胞上清液NO含量；Westernblotting法檢測p-PKCa含量。牛磺酸(40、80和160mmol·L)可抑制AngII誘導(dǎo)的CFb增殖及I型膠原、III型膠原合成增加(P<0．05，P<0．O1)，并使細(xì)胞G。／G．期百分率增加，s期細(xì)胞百分率降低(P<0．05，P<0．O1)。?；撬崦黠@抑制p-PKCa的蛋白表達(dá)，提高CFbNO含量，與AngII組比較差別具有明顯統(tǒng)計(jì)學(xué)意義(P<0
3、．05．P<0．01)。?；撬嵬ㄟ^降低p-PKCa的表達(dá)而提高CFbNO的含量，使CFb的增殖和膠原合成受到抑制。關(guān)鍵詞：?；撬?；心肌成纖維細(xì)胞；一氧化氮；蛋白激酶c中圖分類號(hào)：R963文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：0513—4870(2008)06—0591—05EfectsoftaurineonproliferationofratcardiacfibroblastRENKuang。WANGYan—chun。，，YANGShi—jie(．SchoolofBasicMedicine，JilinMedicalCollege，Jilin132013，China；2．BethuneMedicalCo
4、llege，JilinUniversity，Changchun130021，China)Abstract：Thisprojectaimedtoinvestigatetheeffectoftaurineonnitricoxide(NO)andproteinkinaseCalpha(p-PKCet)intheproliferationofculturedneonatalratcardiacfibroblast(CFb)inducedbyangiotensinII(AngII)，andtoexploretheeffectoftaurineonthesignaltransductionpathw
5、ayinCFbproliferation．TheculturedneonatalratsCFbwereisolatedbytrypsindigestionmethod．TheproliferationofCFbwasinducedbyAngIIanddetectedbythiazoleblue(MTT)colorimetricassay．ThelevelsofcollagenIandcollagenII1weremeasuredbytheELISA．Cellcyclewasanalyzedbyflowcytometry．ThechangeofNOcontentwasmeasuredbyn
6、itricacidreductasemethodandtheproteinexpressofp-PKCetincellswasdeterminedbyWesternblottingtechnology．Amongtheconcentrationof40—160mmol·L～，taurinecouldnotonlypreventthesynthesisofcollagenandtheproliferationofCFbstimulatedbyangiotensinII，butalsoblockCFbintheG0／GlphasefromenteringtheSphase，resulting
7、inmorecellsintheG0／GlphaseandfewerintheSphase(P<0．05，P<0．O1)．TaurinesignificantlyincreasedNOlevelandinhibitedp-PKCetexpressioninCFb(P<0．05，P<0．01)．TheinhibitoryeffectsoftaurineonCFbproliferationandcollagensynthesismigh
