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《探討jwa基因通過mapk信號通路對胰腺癌panc-1細(xì)胞增殖、凋亡、侵襲和遷移過程的作用》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、目的：探討JWA基因通過MAPK信號通路對胰腺癌PANC-1細(xì)胞增殖、凋亡、侵襲和遷移過程的作用。方法：體外培養(yǎng)人胰腺癌PANC-1細(xì)胞，針對JWA基因設(shè)計(jì)干擾RNA(siRNA),并通過脂質(zhì)體轉(zhuǎn)染入細(xì)胞，用MTT法檢測細(xì)胞的堉殖能力、用Transwell實(shí)驗(yàn)檢測細(xì)胞的遷移和侵襲能力。用Westernblot免疫印跡分析檢測JWA及MAPK信號通路的MEK、Erkl/2、JNK、p38三個(gè)通路中相關(guān)蛋白的表達(dá)。結(jié)果：通過MTT法我們發(fā)現(xiàn)JWA蛋白表達(dá)被抑制后，PANC-1細(xì)胞的增殖水平有一定升高，面JWA蛋白表達(dá)下調(diào)后PANC-1細(xì)胞的凋亡水
2、平有一定下降，凋亡相關(guān)蛋白BAX的表達(dá)顯著降低，凋亡抑制基因Bcl-2的表達(dá)有一定的升高。通過Tnmswell實(shí)驗(yàn)我們發(fā)現(xiàn)JWAsiRNA處理后的PANC-1細(xì)胞的穿膜細(xì)胞數(shù)明顯增加，遷移和侵襲能力顯著增強(qiáng)。對于JWA與MAPK通路在胰腺癌細(xì)胞系中的關(guān)系，我們了解到JWAsiRNA組MAPK信號通路中p_MEK和p-Erkl/2蛋白表達(dá)水平有明顯的下降，但MEK、Erkl/2、JNK、p38、p-JNK和p-p38的變化不大。Objective:Thisthesisispreparedwiththeobjectiveofdiscussingt
3、heroleofJWAgeneintheproliferation,apoptosis，invasionandmigrationofPANC-1pancreaticcancercellsviaMAPKsignalingpathways.Methods:HumanPANC-1pancreaticcancercellswerecjilturedin-vitro,andsmallinterferingRNA(siRNA)wasdesignedforJWAgene.Then,siRNAwastransfectintothecellsvialiposo
4、me.CellproliferationwasmeasuredviaMTTassay，cellmigrationandinvasionwasmeasuredviaTranswellchambersandproteinexpressionofJWAandMEK,Erkl/2,JNKandp38ofMAPKwasmeasuredviaWesternblot.Results:ByutilizingMTTwediscoveredthatwhenJWAproteinexpressionwasinhibited,theproliferationofPAN
5、C-1cellswasenhancedtoacertainextent.WhenJWAproteinexpressionwasdown-regjilated，theapoptosisofPANC-1cellswasdecreasedtoacertainextent，theexpressionofapoptosis-associatedprotein(AAP)BAXwassubstantiallydecreasedandtheexpressionofapoptosisinhibitorgeneBcl-2wasenhancedtoacertain
6、extent.ViaTranswellchambers，wediscoveredthatthenumberofpenetratingcellsofPANC-1cellswasincreaseddramaticallyafterthePANC-1cellsweretreatedbyJWAsiRNA,andthemigrationandinvasionofthecellswassubstantiallyenhanced.AstothecorrelationofJWAandMAPKpathwaysinpancreaticcancercells,we
7、cametoknowthatp-MEKandp-Erkl/2proteinexpressionofMAPKpathwayofJWAsiRNAcontrolwasdramaticallydown-regjilated，however，theexpressionofMEK，Erkl/2，JNK，p38，p-JNKandp-p38wassubjectedtoalimitedchange.目的：探討薯蕷皂苷元通過MAPK信號通路處理人H癌BGC-823和SGC-7901細(xì)胞后對其增殖、侵襲、遷移和凋亡過程的影響。方法：體外培養(yǎng)人胃癌BGC-823和S
8、GC-7901細(xì)胞，用薯蕷皂苷元對BGC-823和SGC-7901細(xì)胞進(jìn)行處理，用MTT法、Transwell實(shí)驗(yàn)檢測細(xì)胞的增殖、迂移、侵襲能力。用Westernb
