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1、大豆苷元對(duì)結(jié)扎大鼠冠狀動(dòng)脈所致?lián)p傷的保護(hù)作用作者:葉和楊,胡志蘋,周莉,黃志華,曾靖【關(guān)鍵詞】大豆 ProtectiveeffectofDaidzEinonmyocardialischemiainjuryinrats 【Abstract】AIM:ToobservetheprotectiveeffectofDaidzEInonratsiainjurymodelin.Thirtytaleratslydividedinto4groups:shamoperationgroup,modelgroup,hi
2、ghdose(5.0mg/kg)Daidzeingroupandlog/kg)Daidzeingroup.ThetL/100gthroughsublingualvein10minbeforeischemiainjuryandsalineoperationgroupandmodelgroup.TheleadⅡECGyocardialischemiainjury,15minafterischemiaand30,60,90and120minafterinjectionomentmentionedabove
3、yocardialischemiamodel,thecontentsofmalondialdehyde(MDA)andsuperoxidedismutase(SOD)inmyocardialtissueyocardialinfarctsizeeasuredbyTTCstainingofcardiacmusclecellsinanother24rats(dividedinto3groups:modelgroup,Daidzein5.0mg/kggroup,Daidzein2.5mg/kggroup)y
4、ocardialischemiainjuryinducedbythesamemethods.RESULTS:paredodelgroup,theareaofacutemyocardialinfarctiondeclined,thevalueof∑STreducedandthemaximumvaluesofJpointandTodelgroup.ThecontentofSODarkedlyloodelgroup.Allthechangesmentionedaboveshoedosedependente
5、ffect.CONCLUSION:Daidzeinhasobviousprotectiveeffectonmyocardiumagainstischemiainjurycausedbyligatingthebranchofleftanteriordescendingcoronaryartery. 【Keyyocardialischemia;coronaryarteryligation;serumenzyme;freeradicals 【摘要】目的:觀察大豆苷元對(duì)結(jié)扎大鼠左冠狀動(dòng)脈前降支損傷的保護(hù)
6、作用.方法:取32只雄性DA和SOD值.另用24只DA值減小,SOD值升高,心電圖∑ST值減少,J點(diǎn)和T波的上升的最大值明顯降低,且呈一定劑量依賴性.結(jié)論:大豆苷元對(duì)大鼠結(jié)扎冠狀動(dòng)脈的缺血損傷具有保護(hù)作用. 【關(guān)鍵詞】大豆苷元;心肌缺血;冠脈結(jié)扎;血清酶;自由基 0引言 大豆苷元(Daidzein,DD)系葛根中的主要成份,葛根具有解肌退熱、生津透疹、升陽止瀉的功效[1],其化學(xué)成分復(fù)雜.主要有效成分為葛根素和大豆苷元,現(xiàn)已能人工合成.葛根素的臨床應(yīng)用非常廣泛,對(duì)心腦血管疾?。?-2]、視網(wǎng)膜
7、疾?。?]等具有良好療效;大豆苷元也具有抗心律失常[4]、抑制膽囊收縮[5]、耐缺氧[6]、影響免疫器官[7]等作用.我們通過實(shí)驗(yàn)觀察大豆苷元對(duì)心肌缺血損傷的保護(hù)作用. 1材料和方法 1.1材料健康雄性DA),GSH,乳酸脫氫酶(LDH),磷酸肌酸激酶(CK)試劑盒均購(gòu)自南京建成生物工程研究所.MettlerToledoAG135型光電天平,madeinSg/kg)、大豆苷元低劑量組(2.5mg/kg),n=8.用100g/L水合氯醛(3mL/kg)腹腔麻醉后,氣管插管.左側(cè)第3~4肋間開胸,剪
8、開心包,暴露心臟,于肺動(dòng)脈園錐與左心房之中間找出冠狀動(dòng)脈左前降支,并用圓形無創(chuàng)縫合針6/0絲線置于左冠狀動(dòng)脈前降支起始部下2mm處備用.除假手術(shù)組僅穿線不結(jié)扎外,其余各組均以0號(hào)線立即結(jié)扎.結(jié)扎冠狀動(dòng)脈,以心電圖Ⅱ?qū)?lián)ST段抬高0.1mV或T波高聳、心肌顏色變暗紅色為結(jié)扎成功的標(biāo)志,記錄結(jié)扎前,結(jié)扎15min,給藥后30,60,90,120min的Ⅱ?qū)?lián)心電圖.于結(jié)扎冠狀動(dòng)脈左前降支前10min經(jīng)舌下靜脈注入大豆苷元溶液,缺血模型組及假手術(shù)組經(jīng)舌下靜脈注入等體積生理鹽