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1、萬方數(shù)據(jù)第三軍醫(yī)大學碩士學位論文InⅥtroStudyonInductionofResistancetoFluconazoIeiⅡ乃站廳D置pD陽露口s露辦村BackgrouⅡdandobjectiVeAbstractmc^D點po，_D，zspp．exsitwidelyinnatllre，comprising50species，ofwhichl6speciescancausehumaninfection．Thenumberofinfectionscausedby乃fc^唧D加，zspp．a(chǎn)ccountfor10％ofthedisseminated血ngalinfe
2、ctions．乃f幽cl印f)加，z口s砌ffisthecommoncausatiVeagentof乃f幽。印D加九spp．infections，andcanleadtosystemicandfataltrichosporonOsis，especiallyini姍姒mocompromisedandimmIlrIodeficienthosts，suchaspeoplesuff色ringf．romneutropenia，orgaIltransplaIltation，hematologicmalignancies，extensivebumsorAIDSWiththewid
3、espreaduseofa11tiflmgaldmgs，especiallyofazoles，theanti如ngalresistanceofZ口s口Jlliihasbeenincreasin91yreported．ThishasmadeclinicaldoctorsdimculttodealwithtrichosporOnosis，andthedeVelopmentofnewanti如ngalagentsoRenrequirelongperiod．Therefbre，itissignificaIlttoexplorethenuconazoleresistancem
4、echanismsandfind0utnewt盯getsofanti向ngalagents．Theseneedtoestablishastableaz01e—resistantmodelofZ口J口^“ResistantmodelsacquiredbyinductioninVitroo托npresentlowsuccessrateandinstableresistaIlce，soitisimperatiVetoestablishamethodwhichcanacquirestableresistantstrainssuccess如11y．Inthisstudy，we
5、obtainednuconazole—resistantmodelofr口s砌ifwhichwereculturedinmediumscontainingmethylprednisoloneandnuconazole，andevaluatedthesuccessrateaIldstability．Methods1．16clinicalandenviror吼entalstrainsofZ以J砌村weretestedthesusc印tibilityagainstnuconaz01einVitr0byCLSIM27一A3；2．Basedonthesusceptibilit
6、yresults，thenuconazolesensitiVestrainswereinducedresistancetonuconazolebyindiVidualfluconazole，indiVidualmethylprednisolone，andnuconaz01ecombinatedwithmethylprednis010nerespectiVely，comparingwimthecontr01萬方數(shù)據(jù)grOup；第二軍醫(yī)人學碩士學位論文3．InordertoobserVethestabilityofaz01e-resistalltstrains，isol
7、atesfoundtoexhibitFLCresistancewereseriallycultured30timesindmg-freemedium．A佼ereachsubculture，antifungalsusc印tibilitytestingwasperfomled；4．Inordertomeasurethedevelopmentofcross—resistancetootheranti允ngalagents，thestablenuconaz01e—resistalltstminswereperf．ormedMICsassayswithfourantinm
