資源描述：
《黑莓β-葡萄糖苷酶動(dòng)力學(xué)與其花色苷降解機(jī)理分析》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、Blackberrycontainslargeamountofanthocyaninsandhasbeenapotentialsourceofanthocyaninspigment．Consumersarepayingmoreandmoreattentiontoitsanthocyaninsbecauseoftheirpharmacologicalfunctions．However,Anthocyaninsareveryactiveandunstableunderambientcondition，whichleadstothedeg
2、radationofanthocyaninsduetomanyphysicalandchemicalfactors．Forthesereasons，itisnecessarytoexplorethemechanismofanthocyaninsdegradation，whichCalllayfoundationforkeepinganthacyaninsfrombreakingdownandlosingtheirfunction．Thispaperstudiedtheidentificationandcharacterization
3、ofanthocyaninsusingSPEC18columnandI-IPLC—MS／MSmethod．Theenzymolysiscraftoftheblackberrywasalsostudiedbytheresponsesurfacemethodologybasedonthecontentofanthocyanins．TheeffectsofpH，temperature，solublesolidcontent，enzymes，andVitaminConthedegradationofanthocyaninswerestudi
4、ed．Thedegradationmechanismofanthoeyaninsbyfl-glucosidasewasstudied．Theinactivationkineticsoffl-glucosidasebycombinedhighhydrostaticpressureandtemperaturetreatmentsWasalsostudied．1nheresearchresultsasfollows：(1)TheanthocyaninsinblackberrywereisolatedusingHPLCequii；Iment
5、withalichrospherC18columnandaDADdetectorandcharacterizedbyUVspectrometryandelectrosprayionizationtandemmassspectrometry(ESI—MS／MS)AtotalofsevenanthocyaninswerefoundtobeisolatedbytheC18column．Amongthemwerecyanidin(Cy)3一O—glucoside(68．97％)，Cy3-O-arabi-noside(10．44％)，Cy3一
6、O-malonylglucoside(4．50％)，Cy3一O—dioxalylglucoside(12．40％)，Delphinidin(Dp)3一O-arabinoside(2．20％)，Dp3一O-coumaroyld【ucoside(1．18％)andOp3-O—dioxalylglucoside(0．31％)．(2)Asecondorderpolynomialequationfortheenzymolysiscraftoftheblackberrywasestablishedwithresponsesurfacemetho
7、dologybasedonthecontentofanthocyaninsintheblackberryjuice．Thedataindicatedthattheoptimumenzymolysisparameterswere：amountofEnzyme-0．23％：incubationtime-1．49hours；temperature-46．80C．Undertheseconditions，thecontentofanthocyaninsinblackberryjuiceincreasedby32％，whichwasconsi
8、stentwiththemeasuredvalue．Thedegradationprocessofthepurifiedanthocyaninscanbedescribedbythefh-storderkineticmodel，and
