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《乙肝患者聯合檢測血清前s1、前s2和e抗原的臨床意義》由會員上傳分享，免費在線閱讀，更多相關內容在學術論文-天天文庫。
1、摘要目的：通過比較慢性乙肝患者血清中乙肝病毒(HBV)前S1、前S2抗原、E抗原與HBV-DNA之間的相關性，探討聯合檢測前S1、前S2抗原、E抗原的臨床意義。方法：隨機選擇586份慢性乙肝患者血清樣本，采用雙抗體夾心酶聯免疫吸附試驗(ELISA)檢測患者血清中前S1、前S2抗原和E抗原，采用實時熒光定量聚合酶鏈反應(PCR)法檢測HBV-DNA，比較前S1、前S2抗原、E抗原與HBV-DNA相關性。結果：前S1、前S2抗原、E抗原在HB％DNA陽性組的檢出率明顯高于HBV-DNA陰性組(均P<0．05)，且三者均與HBV-DNA存在相關性(相關系數r分
2、別為0．296，0．174，0．362)，相關性從高到低依次為E抗原、前Sl抗原和前S2抗原。聯合檢測中，前Sl抗原與E抗原、前S2抗原與E抗原、前S1抗原與前S2抗原、三項聯合檢測均與HBV_DNA存在相關性(r分別為O．376，0．589，0．462，0．607)，相關性均高于單項檢測，其中以聯合三項檢測與HBV-DNA相關性最高。結論：前S1、前S2抗原、E抗原與HBV-DNA具有良好的相關性，相關性從高到低依次為E抗原、前S1抗原和前S2抗原，聯合任意兩項及聯合三項檢測相關性均高于單項檢測，且以聯合三項檢測相關性最高。前Sl、前S2抗原、E抗原三
3、項聯合檢測較單項檢測和兩項聯合檢測可以更好判斷乙肝病毒感染復制情況。關鍵詞：前S1抗原；前S2抗原；E抗原；聯合檢測AbstractABSTRACTObjective：TostudytheclinicalsignificanceofcombineddetectionforHBVPre—S1antigen．Pre-S2antigenandHBeAginthepatientswhowereinfectedbyhepatitisBvirus，throughinvestigatingtherelationshipamongHBVPre-S1antigen，Pre
4、-S2antigen．HBeAgandHBV-DNA．Methods：The586serasamplesofchronichepatitispatientswerechosenrandomly．HBVPre-S1antigen．Pre-S2antigenandHBeAgweredetectedbyantibody—sandwichEnzymelinkedImmunosorbentAssay(ELISA)，whileHBV-DNAwasdetectedbyrealtimefluorescencequantitativepolymerasechainreac
5、tion(PCR)．ThentherelationshipamongHBVPre—S1antigen，Pre·S2antigen，HBeAgandHBV-DNAwerestudied．Results：IntheHBV-DNApositivegroup．thedetectabilityratesofHBVPre-S1antigen，Pre—S2antigen，HBeAgweresignificantlyhigherthanthatintheHBV-DNAnegativegroup(allP<0．05)．Moreover,HBVPre-S1antigen,P
6、re—S2antigen，HBeAgwerecloselycorrelatedwithHBV-DNA(r=0．296，0．174，0．362)．TherelationshipbetweenHBeAgandHBV-DNAwasthehighest，thenthatbetweenHBVPre-S1antigenandHBV-DNA．andthatbetweenPre-S2antigenandHBV-DNAwasthelowest．Amongthecombineddetectability,allthecombineddetectabilitygroupsin
7、cludingPre—S1antigenandHBeAgdual-positivegroup，Pre-S2antigenandHBeAgdual—positivegroup．Pre—S1antigenandPre-S2antigendual·positivegroup，Pre-S1antigen，Pre—S2antigenandHBeAgtri-positivegrouphadtherelationgshipwithHBV—DNA(r=O．376，0．589，O．462，0．607)，andalltherelationshipswerehighertha
8、nsingle-positivegroup．IntheHBVPre-S1anti
