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《菠蘿半纖維素多糖提取、純化和免疫活性的研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、摘要為了探求加工廢棄物菠蘿皮渣新的利用途徑，雄文以果汁加工廠的菠蘿皮渣為材料，對(duì)菠蘿畢終維素多耱進(jìn)蟹了提取，臻耀苯酪-虢敦法秘＆l紐．酶滾瓣萁慧耱含量窩爨魚鴦鬣避莓了測(cè)定。菠蘿半纖維索糨多糖PAP4和PAP5的糖含量分別為55．13和60．05％，蛋白禽艇分別為4．75和2．08％。袋罔離予交換和凝膠過濾屢糖技術(shù)對(duì)半纖維粢多糖進(jìn)褥丁癸離純純，褥辯瓣個(gè)純度溜一靛酸梅多糖綴箭PAP4一致和PAP5，玨}。榮掰HPI．C技術(shù)對(duì)箕分子蛩敝單糖組成和含量鮭}行了測(cè)定，兩個(gè)海糖的分予量均犬予2000KD。且都是由木糖和阿拉伯糖
2、兩穆鼙糖夔襄，萁中PAP4-Ii孛囂種耱熬禽鼙努烈蠹漣纜鞠39．32：＆g／g，PAPS-ItP孛兩種耱的含髓分別為68．69和24．53mg／g。對(duì)PAP4．II和PAP5-IIP進(jìn)行了紫外、紅外和核磁燕振撿濺，慰冀鱔穩(wěn)髂惠有了裙步靜了解。采耀棼菸器B港巴餾整增蓬實(shí)驗(yàn)，對(duì)菠爹半繹纜索租多糖組分PAP4和PAP5以及兩個(gè)純度均～組分PAP4-II和PAPS．IIP的免疫活性進(jìn)行丁研究。四個(gè)多耱緞分，對(duì)弘B潺巴纓魏增殪均表現(xiàn)出攤越終靂。在實(shí)驗(yàn)用櫸濃度下，除PAP4-II對(duì)棗鬣t淋琶綴胞增整靜抑制律用與樣品濃度璺弱疹增
3、長關(guān)系井，其余的均存在一個(gè)界限用樣濃度點(diǎn)。當(dāng)樣品濃度小于該用櫸濃度時(shí)，熊抑制作用隨樣鼬濃度的增加麗增強(qiáng)，但當(dāng)槽縣濃魔大予該震搭濃度煮辯，箕簿瀨露露藪掰會(huì)下辯。實(shí)驗(yàn)串避發(fā)褒，在毿樣品濃度簿，PAP5對(duì)B淋巴細(xì)胞的增殖有～定的促進(jìn)作用，但作用并不很強(qiáng)。關(guān)鍵詞：菠蘿半纖維素多糟純化免疫活性AbstractTofindanewwayoftheutilizafionofpineapplepomace，thehemiceUuloscpolysaccharidewasextractedfromthepineapplepomace
4、discardedbysyrupfactor,thecontentsoftotalsugarandproteinweredeterminedusingPenol—VitriolmethodandFolin*Pen01．Method．Thesugarcontent。fthepineapplehemicellulosepolysaecharidePAP4andPAP5wele55．13and60．05％respeetively，andtheproteincontentofthemwere4．75and2．08％．The
5、pineapplehemicelhlosepolysaecharidewaspurifiedusingionchangechromatographyandsizeexclusionchromatography,andtwoacidpolysaceharidesPAP4一IlandPAP5一IIP∽regained．ThemolecularweightofthemwasdeterminedbyHPLC,monosaechaddecompositionandcontentwerealsodeterminedbyHPLC
6、．Themolecularweightofthetwoacidpolysacchaddeswerebothmorethan2000KD，andtheyarebothcomposedofxyloseandarabinose，thecontentsofxyloseandarabinoseofPAP4·IIare54。02and39．32rag／g,andthecontentsofxyloseandarabinosoofPAP5一囂Pwelx：68。69and24．53mg／g．TheUVscan，IRscanandNM
7、RSCAnWeredone，andsomeprimaryinformationaboutthestructureofthepolysaccharidesweregainedfromthem，TheimmunobiologiealactivityofthetworudehemicellulusepolysaccharidesPAP4andPAP5andthetwopurifiedacidpolysaccharidesPAP4-IIandPAP5一IIPweI_estudiedbyTandBlymphcellsexpe
8、riment,andtheyallrestrainedtheincreaseofTandBlyre#cells．Atthemustercontentoftheexperiment，theretrainingeffectofpApa,-IItothemultiplicationofTlymphcellsincreasedfollowingwiththeincr
