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1、摘要為了探求加工廢棄物菠蘿皮渣新的利用途徑,雄文以果汁加工廠的菠蘿皮渣為材料,對(duì)菠蘿畢終維素多耱進(jìn)蟹了提取,臻耀苯酪-虢敦法秘&l紐.酶滾瓣萁慧耱含量窩爨魚鴦鬣避莓了測(cè)定。菠蘿半纖維索糨多糖PAP4和PAP5的糖含量分別為55.13和60.05%,蛋白禽艇分別為4.75和2.08%。袋罔離予交換和凝膠過濾屢糖技術(shù)對(duì)半纖維粢多糖進(jìn)褥丁癸離純純,褥辯瓣個(gè)純度溜一靛酸梅多糖綴箭PAP4一致和PAP5,玨}。榮掰HPI.C技術(shù)對(duì)箕分子蛩敝單糖組成和含量鮭}行了測(cè)定,兩個(gè)海糖的分予量均犬予2000KD。且都是由木糖和阿拉伯糖
2、兩穆鼙糖夔襄,萁中PAP4-Ii孛囂種耱熬禽鼙努烈蠹漣纜鞠39.32:&g/g,PAPS-ItP孛兩種耱的含髓分別為68.69和24.53mg/g。對(duì)PAP4.II和PAP5-IIP進(jìn)行了紫外、紅外和核磁燕振撿濺,慰冀鱔穩(wěn)髂惠有了裙步靜了解。采耀棼菸器B港巴餾整增蓬實(shí)驗(yàn),對(duì)菠爹半繹纜索租多糖組分PAP4和PAP5以及兩個(gè)純度均~組分PAP4-II和PAPS.IIP的免疫活性進(jìn)行丁研究。四個(gè)多耱緞分,對(duì)弘B潺巴纓魏增殪均表現(xiàn)出攤越終靂。在實(shí)驗(yàn)用櫸濃度下,除PAP4-II對(duì)棗鬣t淋琶綴胞增整靜抑制律用與樣品濃度璺弱疹增
3、長關(guān)系井,其余的均存在一個(gè)界限用樣濃度點(diǎn)。當(dāng)樣品濃度小于該用櫸濃度時(shí),熊抑制作用隨樣鼬濃度的增加麗增強(qiáng),但當(dāng)槽縣濃魔大予該震搭濃度煮辯,箕簿瀨露露藪掰會(huì)下辯。實(shí)驗(yàn)串避發(fā)褒,在毿樣品濃度簿,PAP5對(duì)B淋巴細(xì)胞的增殖有~定的促進(jìn)作用,但作用并不很強(qiáng)。關(guān)鍵詞:菠蘿半纖維素多糟純化免疫活性AbstractTofindanewwayoftheutilizafionofpineapplepomace,thehemiceUuloscpolysaccharidewasextractedfromthepineapplepomace
4、discardedbysyrupfactor,thecontentsoftotalsugarandproteinweredeterminedusingPenol—VitriolmethodandFolin*Pen01.Method.Thesugarcontent。fthepineapplehemicellulosepolysaecharidePAP4andPAP5wele55.13and60.05%respeetively,andtheproteincontentofthemwere4.75and2.08%.The
5、pineapplehemicelhlosepolysaecharidewaspurifiedusingionchangechromatographyandsizeexclusionchromatography,andtwoacidpolysaceharidesPAP4一IlandPAP5一IIP∽regained.ThemolecularweightofthemwasdeterminedbyHPLC,monosaechaddecompositionandcontentwerealsodeterminedbyHPLC
6、.Themolecularweightofthetwoacidpolysacchaddeswerebothmorethan2000KD,andtheyarebothcomposedofxyloseandarabinose,thecontentsofxyloseandarabinoseofPAP4·IIare54。02and39.32rag/g,andthecontentsofxyloseandarabinosoofPAP5一囂Pwelx:68。69and24.53mg/g.TheUVscan,IRscanandNM
7、RSCAnWeredone,andsomeprimaryinformationaboutthestructureofthepolysaccharidesweregainedfromthem,TheimmunobiologiealactivityofthetworudehemicellulusepolysaccharidesPAP4andPAP5andthetwopurifiedacidpolysaccharidesPAP4-IIandPAP5一IIPweI_estudiedbyTandBlymphcellsexpe
8、riment,andtheyallrestrainedtheincreaseofTandBlyre#cells.Atthemustercontentoftheexperiment,theretrainingeffectofpApa,-IItothemultiplicationofTlymphcellsincreasedfollowingwiththeincr