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《grim-19在小鼠植入前胚胎中表達(dá)及作用的研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、山東人學(xué)頌：t：學(xué)位論義一個潛在指標(biāo)。3、GRIM．19蛋白的阻斷能影響胚胎的存活率，并阻滯小鼠早期胚胎的發(fā)育，提示GRIM．19在胚胎的早期發(fā)育中發(fā)揮重要作用。關(guān)鍵詞：GRIM．19；線粒體；植入前胚胎；Westernblot；Real—timePCR；顯微注射山東人學(xué)碩．L
2、學(xué)位論文ThestudiesonexpressionofGRIM一19inpreimplantationembryo?！痬iceLnditsfunctionemrOOtmlceanItSUncSpeciality：Gynecologyand
3、ObstetricsPostgraduate：CuiWenjuanSupervisor：Prof．DengXiaohuiProf．ChaoLanABSTRACTPurposesMitochondriaaretherichestoforganellesineggcytoplasm，whicharethemajorsourceofATPgenerationforegg，fertilizedovumandembryo．Duringtheearlydevelopmentofembryo，mitochondriaunderg
4、oaseriesofchangesinmorphologyandlocation．Duringthisstage，littlechangeinmitochondria’Sfunctioncandirectlyinfluencethegrowthandproliferationofpreimplantationembryo．Ifmitochondriarespiratorychaingetimpaired，itwouldcauseATPreduced，aneuploidgenerated，andthedevelopm
5、entpotentialofovumsuppressedduetothedisturbanceofthenormalfertilizationandembryodevelopment．GRIM．19(GenesassociatedwithRetinoid—Interferon-inducedMortality．19)wasfoundtobeapartofcomplexIofmitochondrialrespiratorychainwhichcatalyzedthefirststepofelectrontransfe
6、rintheoxidativephosphorylationsystem(OXPHOS)．AnditsubsequentlywasdemonstratedtobeinvolvedincomplexI—IVassemblyandactivity，andmaintainingthemitochondrialtransmembranepotential(△Y1．GRIM一19?！眀lastocystsstrikinglydisplayabnormalmitochondrialstructure，morphology，an
7、dcellulardistribution．HomologousdeletionofGRIM-19causesembryoniclethalityatemoryomcday9．，．Subsequently．ithasbeendemonstrationthatGRIM-19wasessentialtoearlydevelopmentofheart．However，therewasnoreportabout7山東大學(xué)碩士學(xué)位論文GRIM一19inpreimplantationembryoofmice．So，inthis
8、researchwestudiedthefunctionofGRIM一19inpreimplantationembryoofmiceviadetectingtheexpressionofGRIM一19．MethodS1．SuperoVulationandeggscollectionSexualmaturityfemalemicewereabdominalinjectedwith10IUeachoneofhumanmenopausalgonadotropin(HMG)，and48hlaterinjectedwith1
9、OIUeachoneofhumanchorionicgonadatropin(hCG)．Onefemalemiceandonemalemiceasacouplewerebothplacedtogetherforfertilization．FertilizedovumwerecollectedwithG1mediumthenextday(27hafterhCG
