胡椒堿對大鼠局灶性腦缺血的神經(jīng)保護作用

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1、英文摘要Another32maleSDratsweretreatedassameasitdescribedabove．Alloftheanimalwereperfusedwith4％paraformaldehydeinO．1mol／Lphosphatebufferat24hafterMCAO．Thebrainswereremovedfromtheskull，postfixed24hoursinthesamefixativeandthensectionedinto2．mmcoronalsliceanteriortoopticchiasma．Af

2、terparaffinembedding，theconsecutivesectionswereusedtodetectedapoptosisbyTUNELmethodandexpressionofcaspase一3byimmunohistochemistrytechnologyinthepenumbraregion．Thedatawasdescribedwithmean4-standarddeviation(x士s)．Multiplecomparisonsofthedatawereanalyzedbymulti—sampleOne-WayAN

3、OV久andSNK-qtest．ItwastakenasthesignificantdifferencewhenP<0．05．Results：1．Neurologicdefectappearedinthesaline，vehicleandpiperinegroupratafterMCAO．Therewasnoneurologicimpairmentintheshamedgroup．Therewasnosignificantdifferenceat2hafterMCAOamongthreeofthesaline，vehicleandpiperi

4、negroupp>0．05)．TheNeurologicaldeficitscoresofpiperinegroupwassignificantimprovedcomparedwithitofsalineandvehiclegroupat24h，48hand72hafterMCAO眵

5、16士15．43)mm3inpiperinegroup．Therewasnosignificantdifferencebetween5莢文摘要——————————————————————————————————————一thesalinegroupandvehiclegroupp>0．05)．itwasfoundthatobviousdifferenceofinfarctedvolumesbetweenpiperinegroupandsalinegrouporvehiclegroup(p<0．01)．3．Thecountofapoptosis

6、cellsinsalinegroup，vehiclegroupandpiperinegroupwere52．70-a：6．15，48．58+7．17and20．30-士2。67，respectively．TheapoptosiscellsdecI．easeddramaticallyinpiperinegroup(p<0．01)．4．ThecountofCaspase一3postivecellswere21．30土3．20．22·15+2．57and11．68+1．96insalinegroup，vehiclegroupandplpennegr

7、ouprespectively．Therewasnosignificantdifferencebetweenthesalinegroupandvehiclegroupp>0．05)．TheCaspase-3postivecellsdecreaseddramaticallvinpiperinegroupp

8、otectiveeffectonacuteischemicbraininjuryinrats．2．Thepiperinecaninhibittheapoptosis