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《禽病原性大腸桿菌e058株ompt、cvac基因突變株的構(gòu)建及其生物學(xué)特性》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、禽病原性大腸桿菌E058株ompT、cvaC基因突變株的構(gòu)建及其生物學(xué)特性ConstructionandcharacterizationofompTandcvaCmutantsotavlanPathogemc15scherichiacoli^●1●1—1·11‘本文受國家自然科學(xué)基金(30972196、30771604、30471281)、國家863計劃(2003AA222141)資助研究生：何洪波專業(yè)：預(yù)防獸醫(yī)學(xué)導(dǎo)師：高崧教授劉秀梵教授揚州大學(xué)獸醫(yī)學(xué)院2012年6月何洪波：禽病原性人腸桿菌E058株ompT、n，臼(1基閃突變株的構(gòu)建及其生物學(xué)特性上禽病原性大腸桿菌E058株ompT、cva
2、C基因突變株的構(gòu)建及其生物學(xué)特性；開要三、由㈣y225㈣8803研究生：何洪波揚州入學(xué)碩十學(xué)位論文生株相比沒有明顯的差異，從而表明ompT基因與APECE058株的致病性有關(guān)，cvaC與APECE058株的致病性不相關(guān)。關(guān)鍵詞：禽致病性大腸桿菌；ompT：cvaC；突變株；致病性何洪波：禽病原性人腸桿菌E058株ompT、cva(1基岡突變株的構(gòu)建及其生物學(xué)特性三ConstructionandcharacterizationofompTandcvaCmutantsofaVlanpamogenlcEsc刃ericiqlacoli’1·11T‘1·1‘AbstractM．S．candidate：H
3、eHongboAdvisor：Prof．GaoSongProf．LiuXiufanColibacillosisreferstoanylocalizedorsystemicinfectioncausedentirelyorpartlybyavianpathogenicEscherichiacoli(APEC)，includingsepticaemia，granuloma(Hjarre’Sdisease)，synovitis，airsacdisease／chronicrespiratorydisease(CRD)，peritonitis，salpingitis，aviancellulites，sw
4、ollenheadsyndrome，panophthalmitis，andomphalitis／yolksacinfection．Collectively,infectionscausedbyEscherichiacoli(Ecoli)areresponsibleforsignificanteconomiclossestothepoultryindustryinmanypartsoftheworld．ompTencodesOmpTprotease．a(chǎn)ndcvaCisthestructuralgeneforcolicinVEpidemiologicsurveyshowsthatompTandcv
5、aCareofhighratiointhevirulencestrains．Inthisstudy,therelationshipbetweenompTgeneorcvaCgeneandthepathogenicityofAPECstrainE058wasstudied．AlltheactualrolesoftheknownvirulencefactorsofAPECarenotfullyelucidatedandcertainstepsoftheinfectionprocesshavenotbeenrelatedtopreviouslyidentifiedfactors．Furthermor
6、e，themechanismsbywhichAPECcauseinfectionarelargelyunknown．Inthisstudy，genesinAPECserogroup02strainE058chosenfordeletionwereompTandcvaC．ThefragmentswereamplifiedandsubclonedtopMD⑩18．Tsimplevector．ThevectorT-ompTwasinsertedwithaZeo。genetoformT-ompT-ZeoandT-cvaCwasinsertedwithaZeo。genetoformT-cvaC-Zeo．
7、ThedisruptedompTandcvaCgeneswithZeo。geneinsertedinT-ompT-ZeoandT-cvaC—ZeowereamplifiedbyPCRandpurifiedforelectroporationtoE058competentcells，theresultingZeo‘colonieswereselectedandnamedasE058AompT,E05
