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《mptp致慢性帕金森病小鼠模型的蛋白質(zhì)組學(xué)研究》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、重慶醫(yī)科大學(xué)碩士研究生學(xué)位論文(IPG—IEF)，andthenverticalflatsodiumdodecylsulphatepolyacrylamidegelelectrophoresis(SDS．PAGE)．⑤Theproteinspotsingelswerevisualizedbysilverstainingprotoc01．⑥Scannedgelsandanalyzedtheimages．Searchedforthedifferentiallydisplayedproteinspotsbycomparingtwogrou
2、psofgelimages．QldentifiedsomeofthedifferentiallydisplayedproteinspotswithMS(massspectrometry)followeddigestedwithenzyme．Results：①Behaviorobservationresults：comparedwiththecontrolgroup，thepoleclimbingtimeofthetestgroupwasobviouslylasting(p<0。05)．ThegrouptreatedwithMPTPd
3、isplayedmarkedlyhypoactivebehaviorandthecontrolgroupdidn’tdisplayparkinsonismbehavior．②Throughthecomparisonofthesubstantianigra2-DEmappings(pH3一l0andpH4-7)ofmousebetweenthePDandcontrolgroups，theresolutionof2-DEmappingswithpH4—7IPGDryStripwasbetterthanthosewithpH3．10IPG
4、DryStrip．③Comparingtwoproteinextractionmethodsby2-DEmappings，wefoundthegroupwithnotanytreatmentwasbetter．@ThroughtheanalysisofproteinspotsgroupwithPDQuest8．0analysissoftware，therewere812+21proteinspotsonthesubstantianigra2-DEimagesofPDmouseintherangeofpH4-7，and775+38on
5、thecontrols．18proteinspotswerehyp-expressionand13proteinspotswerehomo—expressiononthesubstantianigra2-DEmappingsofPDbycomparingthemappingsoftWOgroups，twoproteinspotswere7重慶醫(yī)科大學(xué)碩：L研究生學(xué)位論文new．Therewere912士21proteinspotsonthestriatum2-DEimagesofPDpatientsintherangeofpH3—1
6、0，and875士38onthecontrols．6proteinspotswerehyp-expressionand4proteinspotswerehomo—expressiononthestriatum2-DEmappingsofPDbycomparingthemappingsoftwogroups，twoproteinspotswerenew．⑤ThroughtheMSidentifition，thehomo·expressionproteinsspotinsubstantianigrawere0【一enolase．Then
7、ewproteinspotsweretumornecrosisfactorligandsuperfamilymember4andcyclin—dependentkinaseinhibitorlB．Thehomo—expressionproteinspotsinstriatumweremitochondrialfissionregulator1andProteinSI00．A10．ThenewproteinspotswereUbiquitin-likeprotein3precursorandLin．7homologB．Conclusi
8、on：MPTPchronicparkinsonismmodelwasabletorepresentnaturalCouseofParkinson’Sdisease．Theidealsubstantianigra2-DEmappings
