芍藥苷對uvb誘導hacat細胞p53基因和蛋白表達的影響

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1、摘要目的：研究芍藥苷(Paconiflorin)對中波紫外線(UVB)照射誘導HaCaT細胞P53基因和蛋白表達的影響。方法：將HaCaT細胞分為8組：A組：對照組；B組：叭m輻照組；C組：0．25mg／L芍藥苷實驗組；D組：0．5mg／L芍藥苷實驗組；E組：lmg／L芍藥苷實驗組；F組：2．5mg／L芍藥苷實驗組；G組：5mg／L芍藥苷實驗組；H組：10mg／L芍藥苷實驗組。實驗組除A組外，其余組均輻照小m，使用RT-PCR技術(shù)及Western-Blot技術(shù)檢測各處理條件下P53基因及蛋白的表達水平。結(jié)果：與B組比較，各實驗組P53mRNA及P53蛋白的表達量顯

2、著減少(P

3、teinexpressioninHaCaTkeratinocytes．Methods：HaCaTcellsweredividedintoeightgroups：groupA：controlgroup；groupB：UVBradiationgroup；groupC：suppliedwith0．25mg／Lpaeoniflorin；groupD：suppliedwith0．5mg／Lpaeoniflorin；groupE：suppliedwithlmg／Lpaeoniflorin；groupF：suppliedwith2．5mg／Lpaeoniflorin；group

4、G：suppliedwith5mg／Lpaeoniflorin；groupH：suppliedwith10mg／Lpaeoniflorin．ExceptforgroupA,othergroupswereradiatedwithUVB，TheP53mRNAandP53proteinweremeasuredbyRT-PCRandWestern．Blot．Results：ComparedwithgroupB，theP53mRNAandP53proteinexpressionofHaCaTcellsineachexperimentalgroupdecreasedgradu

5、ally(P<0．05)；theP53mRNAandP53proteinexpressionofHaCaTcellsincreasedwiththeincreasingdosages，andgroupCisthelowest．Conclusion：PaeoniflorinhasallprotectiveeffectonphotodamagecausedbyUVBinHaCaTcells,whichCanreducetheexpressionofP53geneandP53protein，andinwhichtheeffectisdecreasedastheconce

6、ntrationofpaeoniflorinisincreasedinacertainrange．Postgraduatestudent：Fanraoqian(DermatologyandVenerology)DirectedbyDr．Hong-QuanChen【l(eywordsJPaeoniflorin；UVBirradiation：HaCaTcells；P53哪7㈣6川5㈣8Ⅲ3洲3洲2洲Y目錄弓l言?????????????????????????????????????????????1第1章材料與方法??????????????????????????

7、??21．1材料???????????????????????????????????????????21．1．1試劑??????????????????????????????????????????21．1．2儀器??????????????????????????????????????????21．1．3工作液配制????????????????????????????一31．2實驗方法??????????????????????????????”41．2．1細胞培養(yǎng)??????????????????????????????41．2．2實驗分組?????

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