資源描述：
《絮凝釀酒酵母的構(gòu)建及其發(fā)酵特性》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫。
1、第59卷第2期化工學(xué)報Vol159No122008年2月JournalofChemicalIndustryandEngineering(China)February2008研究論文絮凝釀酒酵母的構(gòu)建及其發(fā)酵特性1122211王付轉(zhuǎn),沈微,饒志明,諸葛斌,詹曉北,方慧英,諸葛健(1江南大學(xué)工業(yè)生物技術(shù)教育部重點(diǎn)實(shí)驗(yàn)室;2江南大學(xué)生物工程學(xué)院工業(yè)微生物研究中心,江蘇無錫214122)摘要:把S1cerevisiaeW30321A的絮凝基因FLO1和G418抗性基因kanMX接入質(zhì)粒pYX212,構(gòu)建重組質(zhì)粒pYX2FLO2kan并轉(zhuǎn)化S1cerevisi
2、aeZWA46,得到了較強(qiáng)絮凝能力且傳代穩(wěn)定的重組菌ZWA462F2?？疾炱湫跄芰鞍l(fā)酵特性,實(shí)驗(yàn)結(jié)果表明:該重組菌在生長穩(wěn)定期前期啟動絮凝,與葡萄糖的耗竭非耦聯(lián);在初始pH215～610范圍內(nèi)絮凝值無明顯改變;乙醇產(chǎn)量最高達(dá)到816%(體積),乙醇對葡萄糖的轉(zhuǎn)化率達(dá)到其理論轉(zhuǎn)化率的8918%。重組菌ZWA462F2良好的絮凝性能有利于從發(fā)酵液中分離細(xì)胞和細(xì)胞回用,在燃料乙醇工業(yè)生產(chǎn)中有一定的應(yīng)用價值。關(guān)鍵詞:絮凝;轉(zhuǎn)化;發(fā)酵;燃料乙醇中圖分類號:TQ92文獻(xiàn)標(biāo)識碼:A文章編號:0438-1157(2008)02-0437-06Constructi
3、onofaflocculatingyeastforfuelethanolproduction1122WANGFuzhuan,SHENWei,RAOZhiming,ZHUGEBin,211ZHANXiaobei,FANGHuiying,ZHUGEJian(1KeyLaboratoryofIndustrialBiotechnologyofMinistryofEducation2ResearchCenterofIndustrialMicroorganisms,SchoolofBiotechnology,JiangnanUniversity,Wuxi2141
4、22,Jiangsu,China)Abstract:TheexpressionvectorpYX212harboringtheflocculationgeneFLO1fromS1cerevisiaeW30321AandtheG418resistancegenekanMXwastransformedintoS1cerevisiaeZWA461ThetransformantZWA462F2wasobtainedandshowedstrongandstableflocculationability1Otherpropertiesoftherecombina
5、ntstrainwerealsostudied1Theresultsdemonstratedthattheonsetflocculationwasintheearlystationaryphase,notcoincidentwithglucosedepletionintheculturemedium1Moreover,theflocculationabilityofthetransformantshowednodifferenceatinitialpHrangingfrom215to610,andcouldachievethemaximumethan
6、olconcentrationof816%(vol.)withanethanolyieldonglucoseof8918%ofthetheoreticalvalue1TheflocculationpropertyofthetransformantZWA462F2seemedtohavepotentialapplicationinthefuelethanolindustryduetotheeasinesstoseparateandreuseyeastcells.Keywords:flocculation;transformant;fermentatio
7、n;fuelethanol2007-06-06收到初稿,2007-08-16收到修改稿。Receiveddate:2007-06-06.聯(lián)系人:諸葛健。第一作者:王付轉(zhuǎn)(1973—),女,博士研Correspondingauthor:Prof.ZHUGEJian.E-mail:jzhuge@究生,講師。sohu1com基金項(xiàng)目:國家自然科學(xué)基金項(xiàng)目(20676053);國家高技術(shù)Foundationitem:supportedbytheNationalNaturalScience研究發(fā)展計劃項(xiàng)目(2006AA020103);江蘇省青年科技創(chuàng)新人才Fo
8、undationofChina(20676053);theHigh2techResearchand(學(xué)術(shù)帶頭
