胃癌組織中stathmin表達及其與血管生成的關(guān)系

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1、摘要目的探討Stathmin蛋白及mRNA在胃癌發(fā)展過程中的意義及其對血管生成的影響。方法1．使用免疫組織化學(xué)法檢測120例胃癌組織、癌旁組織及30例非腫瘤組織胃黏膜中Stathmin和CDl05的表達，并分析其與胃癌臨床病理參數(shù)之間的關(guān)系，用CDl05標記胃癌組織的微血管并測其密度(MVD)。2．使用實時熒光定量RT—PCR法檢測120例胃癌組織中Stathmin及CDl05mRNA的表達情況。分析StathminmRNA表達與臨床病理學(xué)參數(shù)之間的關(guān)系，并對Stathmin和CDl05進行相關(guān)性分

2、析。結(jié)果i．StathminmRNA和蛋白在胃癌組織中的表達。Stathmin蛋白主要表達于腫瘤細胞胞漿。胃癌組織StathminmRNA和蛋白的表達明顯高于癌旁組織、非腫瘤組織胃黏膜(F=32．82：X2=59．37，P

3、

4、tathmin；CDl05；血管生成；微血管密度；免疫組化；實時熒光定量RT-PCR法AbstractObjectiveToinvestigatetheroleofStathminproteinandmRNAindevelopmentofgastriccarcinomaanditseffectontumorangiogenesis．Methods1。TheexpressionlevelsofStathminproteinandCD105inpathologicalspecimensfrom120ca

5、sesofgastriccarcinomatissue、adjacenttissuesand30casesofnon-tumortissueswereexaminedbyimmunohistochemistry,TherelationshipbetweenStathminexpressionandclinicopathologicalparametersinhumangastriccarcinomaWasanalysed，andthenCD105markedmicrovesseldensity(MV

6、D)WascountedinordertoanalysetherelationshipbetweenStathminexpressionandMVD．2．Usingreal—timefluorescentquantitativeRT-PCRassayfordetectionofStathminandCD105mRNAexpressin120casesofhumangastriccarcinomatissues．TherelationshipbetweenStathminmRNAandclinicop

7、athologicalparametersinhumangastriccarcinomaWasanalysed，，andthenanalysethecorrelationbetweenStathminandCD105expression．Results1．TheexpressionsofStathminmRNAandproteininhumangastriccarcinomatissues．StathminproteinWasprimarilylocalizedinthecytoplasmofthe

8、carcinomacells．Thepositiverateof．Stathminingastriccarcinomawassignificantlyhigherthanthatinadjacenttissuesorinnon-tumortissues(F=32．82；X2=59．37，P<0．05)2．TherelationshipbetweenStathminexpressionandclinicopathologicalparametersinhumangast