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《nedd9在肝癌中的表達(dá)及其與肝癌細(xì)胞生物學(xué)特性之間關(guān)系的研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、分類號R735.7UDC616-006密級公開NEDD9在肝癌中的表達(dá)及其與肝癌細(xì)胞生物學(xué)特性之間關(guān)系的研究盧鵬培養(yǎng)類別全日制學(xué)位類型學(xué)術(shù)學(xué)位一級學(xué)科(專業(yè)類)臨床醫(yī)學(xué)二級學(xué)科(專業(yè))外科學(xué)（普外）研究方向肝癌的基礎(chǔ)與臨床指導(dǎo)教師竇科峰教授（主任醫(yī)師）培養(yǎng)單位西京醫(yī)院肝膽胰脾外科二O一五年五月第四軍醫(yī)大學(xué)博士學(xué)位論文目錄縮略語表·······························································································1中文摘要·················
2、··············································································3英文摘要·······························································································7前言······························································································11文獻(xiàn)回顧·
3、·····························································································13正文······························································································26第一部分NEDD9在人肝細(xì)胞癌組織中的表達(dá)及意義································261材料（包括所涉及的手術(shù)切除標(biāo)本、所用的試劑耗材以及設(shè)備儀器）
4、·······261.1試驗中所涉及的組織標(biāo)本···························································261.2實驗中所用的試劑及耗材···························································261.3實驗中所用到的設(shè)備和儀器························································272方法···············································
5、·············································282.1組織標(biāo)本的采集以及臨床病理資料的獲取······································282.2RT-qPCR方法分析組織細(xì)胞中NEDD9mRNA的表達(dá)水平·················282.3Westernblot檢測組織中NEDD9蛋白的表達(dá)···································302.4免疫組織化學(xué)染色·············································
6、·······················332.5讀片·······················································································342.6隨訪和預(yù)后評估·······································································352.7統(tǒng)計分析·········································································
7、·······353結(jié)果····························································································353.1不同組織中NEDD9mRNA表達(dá)水平的差異···································353.2不同組織中NEDD9蛋白表達(dá)水平的差異·······································363.3NEDD9免疫組織化學(xué)染色····································
8、······················363.
