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1、分類號(hào)Q819學(xué)號(hào)14023019UDC606密級(jí)公開工學(xué)碩士學(xué)位論文紅巨藤壺52kDa膠蛋白的異源表達(dá)與功能研究碩士生姓名曾玲學(xué)科專業(yè)生物醫(yī)學(xué)工程研究方向仿生生物學(xué)指導(dǎo)教師胡碧茹副教授葉宗煌講師國防科學(xué)技術(shù)大學(xué)研究生院二〇一六年十一月ExpressionandFunctionalCharacterizationofthe52kDaCementProtein(cp-52k)inMegabalanusrosaCandidate:ZengLingAdvisor:Prof.HuBiruAdissertationSubmittedinpartialfulfillme
2、ntoftherequirementsforthedegreeofMasterofEngineeringinBiomedicalEngineeringGraduateSchoolofNationalUniversityofDefenseTechnologyChangsha,Hunan,P.R.China(November,2016)國防科學(xué)技術(shù)大學(xué)研究生院碩士學(xué)位論文目錄摘要.............................................................................................
3、................................iABSTRACT..................................................................................................................ii第一章緒論................................................................................................................11.1研究背景
4、及目的意義......................................................................................11.2國內(nèi)外研究現(xiàn)狀..............................................................................................31.2.1藤壺簡介............................................................................
5、....................31.2.2藤壺膠的成分與功能研究....................................................................61.2.3藤壺膠的交聯(lián)固化機(jī)制......................................................................101.2.4藤壺52kDa膠蛋白..........................................................................
6、..121.3本課題主要研究內(nèi)容....................................................................................17第二章大腸桿菌表達(dá)紅巨藤壺52kDa膠蛋白...................................................202.1紅巨藤壺Mrcp-52k基因的獲取..................................................................202.1.1Mrcp-52k基因密碼子
7、偏好性分析......................................................212.1.2Mrcp-52k基因的優(yōu)化與合成..............................................................212.2Mrcp-52k基因載體的構(gòu)建............................................................................242.2.1PCR擴(kuò)增Mrcp-52k基因片段..................
8、..........................