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1、園藝學報，2016，43(2)：365–372.ActaHorticulturaeSinicadoi：10.16420/j.issn.0513-353x.2015-0590；http：//www.ahs.ac.cn365山東省芍藥紅斑病病原菌鑒定1121,*李麗，宋淑香，劉會香，郭先鋒12（山東農業(yè)大學林學院，山東泰安271018；山東農業(yè)大學植物保護學院，山東泰安271018）摘要：從山東菏澤和泰安兩地感病芍藥（PaeonialactifloraPall.）植株的葉片上進行了紅斑病病原分離純化和致
2、病力檢測，并對致病菌進行了形態(tài)學鑒定及rDNA-ITS序列分析。研究結果表明，病樣組織中可分離到兩種疑似病原菌，分離比例為4.8︰1，初步鑒定為鏈格孢（Alternariaalternata）和細極鏈格孢（A.tenuissima）。進一步rDNA-ITS分析結果表明，兩種病原菌5.8SrDNA及其兩側ITS區(qū)序列分別與GenBank中序列號為KF380822.1、HM467832.1的同源性最高，均為99%，由此確定兩種病原菌分別為鏈格孢Alternariaalternata和細極鏈格孢A.ten
3、uissima，其序列已在GenBank登錄，登錄號分別為KR912224和KR912225。因此，確定山東省芍藥紅斑病是由鏈格孢A.alternata和細極鏈格孢A.tenuissima兩種病原菌引起的復合侵染。關鍵詞：芍藥；紅斑病；病原鑒定；鏈格孢屬；rDNA-ITS+中圖分類號：S682.12文獻標志碼：A文章編號：0513-353X（2016）02-0365-08IdentificationofRedSpotPathogensonPeonyinShandongProvince1121,*LI
4、Li，SONGShu-xiang，LIUHui-xiang，andGUOXian-feng12（CollegeofForestry，ShandongAgriculturalUniversity，Tai’an，Shandong271018，China；CollegeofPlantProtection，ShandongAgriculturalUniversity，Tai’an，Shandong271018，China）Abstract：Redspothasbeenoneofthemostseriousd
5、iseasesinherbaceouspeonyproduction.Identificationofthepathogencausingthediseaseisnecessaryforthedeterminationofeffectivetreatmentoptions.Inthepresentstudy，firstly，thefungiwereisolatedandpurifiedfromtheinfectedleavesandthepathogenicitywasdetectedbyartif
6、icialinoculation.ThenthemorphologicalcharacteristicsandrDNA-ITSsequenceofthepathogenscausingredspotdiseasewasstudied.Theresultsdemonstratedthattwokindsofsuspectedfungi，AandB，wereisolatedfromthedisease-infectedsamples，withtheisolationratioof4.8︰1.Bothfu
7、ngiwerefurtherconfirmedpathogenicandthereforedeterminedasthepathogens.ThemorphologicalcharacteristicsrevealedthatthesetwopathogenswereAlternariaalternateandA.tenuissima，whichwasvalidatedbyfurtherrDNA-ITSanalysis.ITS1-5.8SrDNA-ITS2sequencesofpathogenAan
8、dpathogenBwererespectivelyhighlyhomogenicwiththoseofKF380822.1andHM467832.1inGenBankdatabase，hencetheywererespectivelyidentifiedasA.alternateandA.tenuissima.ThesequencesofthesetwopathogenshavebeensubmittedtoGenBank，andtheaccessionnumber
