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1、園藝學報,2016,43(2):365–372.ActaHorticulturaeSinicadoi:10.16420/j.issn.0513-353x.2015-0590;http://www.ahs.ac.cn365山東省芍藥紅斑病病原菌鑒定1121,*李麗,宋淑香,劉會香,郭先鋒12(山東農業(yè)大學林學院,山東泰安271018;山東農業(yè)大學植物保護學院,山東泰安271018)摘要:從山東菏澤和泰安兩地感病芍藥(PaeonialactifloraPall.)植株的葉片上進行了紅斑病病原分離純化和致
2、病力檢測,并對致病菌進行了形態(tài)學鑒定及rDNA-ITS序列分析。研究結果表明,病樣組織中可分離到兩種疑似病原菌,分離比例為4.8︰1,初步鑒定為鏈格孢(Alternariaalternata)和細極鏈格孢(A.tenuissima)。進一步rDNA-ITS分析結果表明,兩種病原菌5.8SrDNA及其兩側ITS區(qū)序列分別與GenBank中序列號為KF380822.1、HM467832.1的同源性最高,均為99%,由此確定兩種病原菌分別為鏈格孢Alternariaalternata和細極鏈格孢A.ten
3、uissima,其序列已在GenBank登錄,登錄號分別為KR912224和KR912225。因此,確定山東省芍藥紅斑病是由鏈格孢A.alternata和細極鏈格孢A.tenuissima兩種病原菌引起的復合侵染。關鍵詞:芍藥;紅斑病;病原鑒定;鏈格孢屬;rDNA-ITS+中圖分類號:S682.12文獻標志碼:A文章編號:0513-353X(2016)02-0365-08IdentificationofRedSpotPathogensonPeonyinShandongProvince1121,*LI
4、Li,SONGShu-xiang,LIUHui-xiang,andGUOXian-feng12(CollegeofForestry,ShandongAgriculturalUniversity,Tai’an,Shandong271018,China;CollegeofPlantProtection,ShandongAgriculturalUniversity,Tai’an,Shandong271018,China)Abstract:Redspothasbeenoneofthemostseriousd
5、iseasesinherbaceouspeonyproduction.Identificationofthepathogencausingthediseaseisnecessaryforthedeterminationofeffectivetreatmentoptions.Inthepresentstudy,firstly,thefungiwereisolatedandpurifiedfromtheinfectedleavesandthepathogenicitywasdetectedbyartif
6、icialinoculation.ThenthemorphologicalcharacteristicsandrDNA-ITSsequenceofthepathogenscausingredspotdiseasewasstudied.Theresultsdemonstratedthattwokindsofsuspectedfungi,AandB,wereisolatedfromthedisease-infectedsamples,withtheisolationratioof4.8︰1.Bothfu
7、ngiwerefurtherconfirmedpathogenicandthereforedeterminedasthepathogens.ThemorphologicalcharacteristicsrevealedthatthesetwopathogenswereAlternariaalternateandA.tenuissima,whichwasvalidatedbyfurtherrDNA-ITSanalysis.ITS1-5.8SrDNA-ITS2sequencesofpathogenAan
8、dpathogenBwererespectivelyhighlyhomogenicwiththoseofKF380822.1andHM467832.1inGenBankdatabase,hencetheywererespectivelyidentifiedasA.alternateandA.tenuissima.ThesequencesofthesetwopathogenshavebeensubmittedtoGenBank,andtheaccessionnumber