烏賊墨中抗菌及抗腫瘤生理活性物質(zhì)的研究

《烏賊墨中抗菌及抗腫瘤生理活性物質(zhì)的研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。

1、上海水產(chǎn)大學(xué)碩士學(xué)位論文烏賊墨中抗菌及抗腫瘤生理活性物質(zhì)的研究姓名：杜鐵平申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：水產(chǎn)品加工及貯藏工程指導(dǎo)教師：周培根2003.5.16摘要本文主要側(cè)重從烏賊加工副產(chǎn)物的和用角度磁發(fā)，對(duì)鳥賊墨中的生攜活性嬲矮進(jìn)行研究，以裳為烏賊加工中廢棄物的綜合利朋提供線索。重煮磷究了烏賊墨中婉辮瘸活贛耪霞靜提取及冀抗瓣瘸活毪和烏賊墨申撬煎活牲物質(zhì)的提取及其抗藤活性。第一部分為鳥賊蹩蒸本成分的確定和烏賊墨率騷自多糖(PGs)純他及摭腫瘤活性研究。烏賊墨縫脫脂制成丙酮干粉，然后采糟Tris-HCl緩洚溶液播攆，通過(guò)燕交試驗(yàn)

2、虢定提取互嫠，焉提取盼糨蟹是多糖對(duì)腫瘤纓胞傲體外細(xì)胞毒實(shí)驗(yàn)，結(jié)果表明烏賊墨提取物蛋自多糖對(duì)黑人窗頸癌細(xì)脆無(wú)明顯的髂外緇脆毒作瘸。第二部分對(duì)烏賊墨抗菌活性進(jìn)行了研究。烏賊墨中抗菌活性物震麓提敢分離方法，裰?jǐn)fMoehizuki翁方法藕侔改變。烏賊墨中挽菌活性物質(zhì)為黃色吸潮性粉寒，該物成的褥率為O。52％。對(duì)大腸桿菌和金黃色葡萄球菌有摔菌作用，并對(duì)其攘菌特性遴行研究；將該提取物與溶菌酶的部分性質(zhì)進(jìn)行比較，得出該提取物與溶菌酶性覆存在鼴著差異；將該提取裙進(jìn)行鑫罷櫥，發(fā)璦該提玻物主要包含兩個(gè)組分。關(guān)鍵謠：烏賊墨；抗胖瘸活性；抗菌活

3、性；分離；提取上海水產(chǎn)大學(xué)碩士學(xué)位論文AbstractThisprojectwasconcemedabouttheutilizationofcuttlefishinkwhichisthebyproductofcuttlefishprocessing．Thebioactivtialsubstancesofcuttlefishinkwerestudied．A11theseworkweredonetofindawaytogooduseofthewasteofcuttlefishprocessing．Thepaperismain

4、lycomposedoftwopartsFirstly,theessentialcontentsofcuttlefishinkweredetermined，thePGswerepurifiedandpreparedfromcuttlefishinkandtheantitumorailcharactersofthissubstancewerestudiedinthefirstpartofthispaperThecuttlefishinkwasdegreasedinacetoneandablackpowderwasobtai

5、ned。ThentheblackpowderwasextractedwithTris-Helbuffer,theorthogonaltrailwasdonetodetermineextractionprocessing．ThecrudePGspowderwasobtainedandantitumorexperimentontumorcellsinvitrowasdone，itwasfoundthatthePGsdidnotshowthedirectcytotoxicactivity．Theantibacterialeff

6、ectoftheextractfromink—sacofcuttlefishonbacteriawasstudiedinthispart．TheextractionmethodwasmodifiedbasedonthatofMochizuki。Theextractwasyellowhygroscopicpowderanditsyieldobtained0．52％．TheextracthadtheantibacterialeffectonStaphk’lococcusaureusandEscherichiacoli．Som

7、eofthecharactersoftheantibacterialsubstancewerestudied．Comparingthissubstancewithlysozyme，itwasfoundthatthedifferentbetweenthemwasinevidence上海水產(chǎn)大學(xué)碩士學(xué)位論文Thispowd囂wasfilterbyDEAESepharoseCL-6Bgelcolumn，twomainlyfractionswereobtained。Keywords：cuttlefishink；antitoumo

8、r；antibacterial；separation；extractionJ二海水產(chǎn)大學(xué)碩士學(xué)位論文日U孬烏賊(Cuttlefish)屬于軟體動(dòng)物f](肋““mMollusca)頭足綱((：lassCephalopode)鳥賊日(SepioideaⅣcfi力烏賊科(SepidacKi磚rtein)烏賊屬(SepiaL