含神經(jīng)生長因子的羊膜基質(zhì)管橋接修復(fù)神經(jīng)缺損的實驗研究

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1、華中科技大學(xué)博士學(xué)位論文含神經(jīng)生長因子的羊膜基質(zhì)管橋接修復(fù)神經(jīng)缺損的實驗研究姓名：梁群申請學(xué)位級別：博士專業(yè)：外科學(xué)骨科指導(dǎo)教師：陳安民2003.4.1華中科技大學(xué)同濟醫(yī)學(xué)院博士畢業(yè)論文含神經(jīng)生長因子的羊膜基質(zhì)管橋接修復(fù)神經(jīng)缺損的實驗研究華中科技大學(xué)同濟醫(yī)學(xué)院附屬同濟醫(yī)院研究生：梁群導(dǎo)師：陳安民教授摘要目的研究含神經(jīng)生長因子(nervegrowthfactor，NGF)的人羊膜基質(zhì)(humanammionmembranematrix，HA刪)微孔管(HA刪+NGF)橋接修復(fù)周圍神經(jīng)缺損的作用和效果。方法將健康Wistar大鼠80

2、只按治療方法隨機分為分為4組，每組每時段8只，分別用含神經(jīng)生長因子人羊膜基質(zhì)微孔管(HA涮+NGF)、人羊膜基質(zhì)微孔管(HAMM)、神經(jīng)生長因子(NGF)及生理鹽水對照修復(fù)坐骨神經(jīng)1．0cm缺損；術(shù)后4、8周和12周分別進行傷肢功能恢復(fù)測定、電生理學(xué)、組織形態(tài)學(xué)檢查、圖像分析和透視電鏡觀察。結(jié)果近端神經(jīng)再生軸突生長通過1．5cm長的HAE*+NGF移植體，重新支配終末骨骼肌，其再生神經(jīng)纖維生長速度、數(shù)量明顯快(多)于單純HA涮移植體和單純的NGF組，術(shù)后12周時坐骨神經(jīng)功能恢復(fù)良好。結(jié)論HAMM和NGF聯(lián)合使用可促進軸突再生及其

3、髓鞘化，對損傷神經(jīng)及傷肢的功能恢復(fù)有明顯協(xié)同促進作用，HAMM+NGF是一種很有希望的促進周圍神經(jīng)再生的生物材料。關(guān)鍵詞周圍神經(jīng)神經(jīng)再生神經(jīng)生長因子人羊膜基質(zhì)竺!型彗查堂旦塑墾蘭墮———————————————j生蘭蘭些蘭苧—一ExperimetalstudiesofperiphealnerveregenerationthroughHAMM+NGFgraftsinratsDepartmentofOrthopaedicSurgery,TongjingHospital，TongjiMedicalCollege，HuazhongUni

4、versityofScienceandTechnologyPostgratuateCandidate：Supervisor：Prof．CHENAbstactLIANGQunAnminObjectiveToinvestigatetheeffectregenerationthroughHA刪+NGF(humanammionnervegrowthfactor)grafts．ofperipheralnervemembranematrixandMethods80Wistarratswererandomlydividedintofourgr

5、oupscontrolgroup，+NGFgroup，HAMMgroupandHAYN+NGFgroup，with8ratsineachandineachperiodoftime．A1．0cmdefectoftheleftsciaticnerveweremadeandthenrepairedrandomlybydifferentmeans．Histomorphometryexaminationwasundertakenat4，8weekspostoperatively．At8，12weekspostoperation，thefo

6、llowingitemsasfunctionalassessmentsofend—organreconnection，electrophysi。logy，imageanalyseandtransmissionelectronmicroscopeobservationweredeterminedResultsAt12weekspostoperation，MotornerveconductionvelocityofthenerverepairedwithHAMMorNGFwerelowerthanthat蘭!型墊查蘭墮墮墮堂墮堡圭蘭

7、些墮塞——一ofthenerverepairedwithheterograftofHAMM+NGF．At12weekspostoperation，HAMM+NGFconduitgroupshowedmorenervefibers，biggeraxondiameterandthickermeylinsheathsthanothergroupS．ConclutionThecombinationofHA塒andNGFwouldenhancenerveregenerationandmyeliogeny，aswellascouldcoop

8、erativelypromotethefunctionalrecoveryofinjurednervesandlimbs．OurresultsindicatethattheHAMM+NGFmicroporousconduitiSapromisingtoolfor