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1、WorldJMicrobiolBiotechnol(2010)26:2163–2170DOI10.1007/s11274-010-0400-0ORIGINALPAPERSelectiveisolation,evaluationandcharacterizationofantagonisticactinomycetesagainstRhizoctoniasolaniHemantJ.Patil?AlokK.Srivastava?SudheerKumar?BhushanL.Chaudhari?DilipK.Aro
2、raReceived:12February2010/Accepted:22March2010/Publishedonline:2April2010óSpringerScience+BusinessMediaB.V.2010AbstractThebaitingbagmethodwasfoundtobeusefulfornewbioactivesubstances.About61%ofallbioactiveforisolatingantagonisticactinomycetesfromterrestrial
3、microbialmetaboliteswereisolatedfromactinomyceteshabitat.Outoftotal110actinomycetesisolatedfromrhizo-(Monchevaetal.2002).Mostcommonantibioticproducingsphericandnon-rhizosphericsoilofIndoGangeticPlainssoilmicroorganismsbelongtothegenusStreptomyces;(IGP)ofIn
4、dia,9isolatesexhibitedaggressiveantagonismabouttwo-third([4,000)ofnaturallyoccurringantibiotics,againstRhizoctoniasolani,screenedthroughdualculture,suchasaminoglycoside,anthracyclines,chloramphenicol,welldiffusionandsealedplatetechnique.Maximumgrowthb-lact
5、ams,macrolides,andtetracyclines,amongothers,hasinhibitionwasrecordedupto50%underwelldiffusion(S.beendiscoveredfromStreptomycesstrains(Goodfellowtoxytricinivh22)and52.6%inadirectconfrontation(Acti-etal.1988).Theproductionoflargenumberofantimicrobialnomyceta
6、lesbacteriumvh41).WhereasmaximumdiseasecompoundsbyStreptomycesspp.(Berdy2005)andantag-suppression(53.33%)undergreenhouseconditionwasonisticimpactsofactinomycetesonpathogenicfungiareachievedonseedlingtreatedwithS.tricolorvh85.Scanningwelldocumented,butfewsp
7、ecieshavebeenimplicatedinelectronmicroscopyofantagonistsandpathogeninteractionbiologicalcontrolofplantdiseases(Bressan2003).exhibitedporeformationandhyphaldegradationoftestExceptafewdeeplystudiedactinomycetestaxa,otherspathogen.Physiologicalandmolecularcha
8、racterizationofaredif?culttobeisolatedfromsoilbycommonisolationselectedisolatesshowedwidediversityanduncommontechniquesbecauseofitsslowgrowthandspeci?crequire-specieshasbeenencounteredthroughtheselectiveisola
