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1、中國水稻科學(ChinJRiceSci),２０１５,２９(１):９７－１０５http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０１．０１２９７水稻苗期紋枯病抗性鑒定微室接種技術的改良徐國娟１,２袁正杰２左示敏３潘學彪３王志龍４瞿紹洪２,?(１浙江師范大學化學與生命科學學院,浙江金華３２１００４;２浙江省農(nóng)業(yè)科學院,病毒學與生物技術研究所,杭州３１００２１;３揚州大學江蘇省作物遺傳生理重點實驗室/教育部植物功能基因組學重點實驗室,江蘇揚州２２５００９;４湖南農(nóng)業(yè)大學湖南省作物種質(zhì)創(chuàng)新與資源利用重點實
2、驗室,長沙４１０１２８;?通訊聯(lián)系人,EＧmail:squ＠m(xù)ail．zaas．a(chǎn)c．cn)ImprovementoftheMicroＧchamberInoculationMethodforDeterminationofRiceSeedlingResistancetoSheathBlight(Rhizoctoniasolani)XUGuoＧjuan１,２,YUANZhengＧjie２,ZUOShiＧmin３,PANXueＧbiao３,WANGZhiＧlong４,QUShaoＧhong２,?１(CollegeofChemistryandLifeScience
3、s,ZhejiangNormalUniversity,Jinhua３２１００４,China;２InstituteofVirologyandBiotechnology,ZhejiangAcademyofAgriculturalSciences,Hangzhou３１００２１,China;３KeyLaboratoryofCropGeneticsandPhysiologyofJiangsuProvince,CollegeofAgriculture,YangzhouUniversity/KeyLaboratoryofPlantFunctionalGenomicsof
4、theMinistryofEducation,Yangzhou２２５００９,China;４HunanProvincialKeyLaboratoryofCropGermplasmInnovationandUtilizationandCollegeofAgronomy,HunanAgriculturalUniversity,Changsha４１０１２８,China;?Correspondingauthor,EＧmail:squ＠m(xù)ail．zaas．a(chǎn)cc．n)XUGuojuan,YUANZhengjie,ZUOShimin,etal．Improvementof
5、themicroＧchamberinoculationmethodfordeterminationofriceseedlingresistancetosheathblight(Rhizoctoniasolani)．ChinJRiceSci,２０１５,２９(１):９７Ｇ１０５．Abstract:Thesheathblightresistanceinricebelongstoatypicalquantitativetrait,andmostofthericecultivarsshowlowlevelsofresistancetothediseasewithsm
6、alldifferences．Theinoculationandscoringsystemforricesheathblightisanimportantbasisforbreedingresistantricecultivars．ThemicroＧchambermethodfordeterminationofriceresponsestothesheathblightpathogenRhizoctoniasolaniwasimprovedbyinoculationtoriceseedlingswithsimilargrowthvigourundercon
7、trolledillumination,temperatureandhumidityconditionsinaplantgrowthchamber．ThericecultivarsYSBR１,Teqing,Taijing３９４,NipponbareandLemontshowedhightolowlevelsofsheathblightresistance,whichwasconsistentwiththeresultsoffieldinoculationinadultriceplants．RTＧPCRanalysisshowedthattheexpress
8、ionoffourricegenesrelatedtodiseas
