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1、萬(wàn)方數(shù)據(jù)Acm冊(cè)q印如s如姍函d蜘如n200I,27(s):387~392387Ef托ctof02,ACCandC02ConcentrationontheActiVityofPartiallyPuri6edACCoxidasefromPapayasONGso“g—Quanl,2”wing—KinYip2(17heK“kbo川o,y礦&月en螂7Ⅲw曠Ⅳ【『lmv礦E血cⅡfm“,sckDf礦L沿&正,"es,刀【m夠^曲№‰咿,Gu北膽hou51【】275;20印“n刪Ⅲ訂日ofⅡHy,om礎(chǔ)5町o,日m29KDr蠟,P。UikmR。耐,Hong
2、Kong)Abstract:l—Aminocyclopmpanc一1一carboxlicacid(ACC)isox訊ized協(xié)fonnethvknebvACCoxidaseThep州i“pu打6cationofACCoxidasefromhiLpeels0fp印8yaa∞deffecfsofsubstrales(“ygⅫandACC).cofacLors(CO,andFe“)andinhibitors(Co¨and眥一aminoisobutvricacid)onmoz£roethvI—encp1吣ductionratewereinve札i冀8te
3、d.ACCo婦dasehasbee”purined19.5—foldbyusi”gDEAE—Sepharoseandne”y1一S。phamsecolumns.11heK。valuebyACCoxidascfor02ine山y(tǒng)lenepmductionva^edgrea【lyde—pendingonIheACCconcen岫don,anddecreascd¨i【}IincreaseoI_ACClevels.TheKvalueforACCal—sodeclinedasox坩肆nconc咖frationinc腫8sed.Theenzvmeacllvi
4、”wasdr日m“calhincreasedbvCO,,andtheK。vaIuefor0,andforACCincreasedwithincreaseofCO,concentra“onEnzvmc8ctivitieswercohvioudvincreasedbvFe¨andsignincandvinhibitedb~C02_;andFe¨couldanl日gonizefl】ejIlhmiiowroJeofC02?!痳hesekineficdataaIeconsistentwith血evjew出afIheca婦lv£icf日l(shuí)eofACCo秭da
5、sefollowsanordeledbindi“gmechanismlnwhichACCoxidasebinds耵rsftoO,a王ldthentoACC.Keywords:ACCoxidasc,&r婦腳。弘k,CO,,F(xiàn)c¨,in—11lblLor.K。vallle,p州ialp11nnc“o”Thcethylenep18ysanimpo^antr0Ieinmany8s—pectsDfp‰tgmw血anddevelopment,includingfnJitT1peningandsenescence(Abelesd“z,1992).1’henn剞
6、stepofel}lvIenebiosvnt}Ie《siscaLalvzedbvthecnzvmeACCoxidasefformedyknownas出ee出ylene一凡rmingenzvme).InsomccasesAOCoxidasealsop18ysanimportanttDIein血eregu【ation“ethyleneproduction,eginHlclon(Lasscrree£口f.1996),fomat0(BaⅡY以n2.1996)andRM,船zpoZ瑚旆Sm(vnezen出Ⅱf.1999).OwinEto山e{actthat
7、ACCoxldase神tiv畸wascompk【clvlostuponhomogeni一盟lio”of£1ssue.itwasonlv胡er山ediscovew£ba£i£sm毗r。aclivi嬸requircsascorbateandF一+thatpu打6一catlonandsubscⅡuentcharacterizationofthisenzvmehasbecomepossible(Ven.eridisandJohn199I).ACCoxidascactivitvhasbcens七Lldiedinseveralcli—mactericfhit
8、ssuchasmelon(Smith“口f.1992),avocado(Mccarv。yandChrist(,飪brsen1992),a