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1、生物工程學(xué)報(bào)ChinJBiotech2010,January25;26(1):1?8journals.im.ac.cnChineseJournalofBiotechnologyISSN1000-3061cjb@im.ac.cn?2010CJB,Allrightsreserved.綜述植物同源結(jié)構(gòu)域指蛋白在擬南芥等十字花科植物春化作用途徑中的功能胡功鈴，陳國平，胡宗利，顧峰，李勇重慶大學(xué)生物工程學(xué)院，重慶400030摘要:春化低溫處理可以使擬南芥等十字花科植物提前開花，該過程中涉及到一個(gè)重要的植物同源結(jié)構(gòu)域指(PHD-finger)蛋白VERNALIZATIONINSENSITIVE3(VI
2、N3)。PHD-finger結(jié)構(gòu)域是真核生物中一種進(jìn)化保守的鋅指結(jié)構(gòu)域，通常參與蛋白質(zhì)之間的相互作用，特別是對核小體組蛋白進(jìn)行甲基化、乙?；?、磷酸化等修飾。在春化處理過程中，VIN3及其同源基因編碼的蛋白都具有PHD-finger結(jié)構(gòu)域，該結(jié)構(gòu)域通過對開花抑制基因FLOWERINGLOCUSC染色質(zhì)組蛋白進(jìn)行H3K9、H3K27甲基化、H3K9和H3K14去乙?；刃揎?，調(diào)節(jié)FLC染色質(zhì)結(jié)構(gòu)狀態(tài)，使其從松弛狀態(tài)轉(zhuǎn)變?yōu)楦叨饶s狀態(tài)而關(guān)閉其功能，從而影響FLC轉(zhuǎn)錄活性進(jìn)而促進(jìn)開花。以下綜述了擬南芥等十字花科植物春化作用途徑中PHD-finger蛋白的功能，并且概述了春化作用機(jī)制。關(guān)鍵詞:植物同
3、源結(jié)構(gòu)域指蛋白，VIN3，F(xiàn)LOWERINGLOCUSC，春化作用，組蛋白修飾Functionofplanthomeodomain-fingerproteinsinvernalizationpathwayinArabidopsisandothercruciferousplantsGonglingHu,GuopingChen,ZongliHu,FengGu,andYongLiBioengineeringCollegeofChongqingUniversity,Chongqing400030,ChinaAbstract:VernalizationmakesArabidopsisandother
4、cruciferousplantsfloweringearlier.Duringthisprocess,animportantplanthomeodomain-finger(PHD-finger)proteinnamedVIN3isinvolved.ThePHDdomainwasaconservedzinc-fingerdomainineukaryoticorganism.Itusedtotakepartintheinteractionbetweenproteins,especiallythemodificationonhistoneofnucleosome,suchasmethylati
5、on,acetylationandphosphorylation.Invernaliazationpathway,theproteinstranslatedbyVERNALIZATIONINSENSITIVE3(VIN3)andhomologousgenescouldresultinmethylationonH3K9andH3K27anddeacetylationonH3K9andH3K14onchromatinhistoneofFLOWERINGLOCUSC,agenethatinhibitedflowering.ThestructurestateofFLCwouldbechangedf
6、romrelaxationintocompression.ThenthetranscriptionactivityofFLCcouldberestrainedanditcouldn’tinhibitfloweringanymore,soitwouldinducefloweringearlier.ThispaperreviewedthefunctionofPHD-fingerproteinsinvernalizationpathwayinArabidopsisandothercruciferousplants,andoverviewedthevernalizationmechanism.Ke
7、ywords:planthomeodomain-fingerproteins,VIN3,FLOWERINGLOCUSC,vernalization,histonemodificationReceived:September3,2009;Accepted:November11,2009Supportedby:NationalNaturalScienceFundationofChina(Nos.30771464,308717
