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1、第二軍醫(yī)大學(xué)學(xué)報2000May;21(5)·456·AcadJSecMilMedUniv·論著·【文章編號】0258-879X(2000)05-0456-04人肝癌裸鼠皮下-肝原位移植瘤模型的建立鄭建明,陶文照,鄭唯強,寶建中,王彥麗,朱明華【摘要】目的:建立人肝癌裸鼠皮下-肝原位移植瘤模型。方法:先用組織學(xué)完整的新鮮人肝癌組織接種于裸鼠皮下,形成皮下移植瘤,然后用此移植瘤組織再接種于裸鼠肝內(nèi),建立肝原位移植瘤模型(間接肝原位移植瘤模型),并將其與直接肝原位移植瘤模型、皮下移植瘤模型和腹腔內(nèi)移植瘤模型作比較。結(jié)果
2、:建立的間接肝原位移植瘤模型能穩(wěn)定傳代,移植瘤存活率達100%,自發(fā)轉(zhuǎn)移率達57.8%。與直接肝原位移植瘤模型比較,該模型移植瘤存活率明顯高于后者(35.6%,P<0.01);與皮下移植瘤模型和腹腔內(nèi)移植瘤模型比較,該模型局部侵襲率(100%)和自發(fā)遠處轉(zhuǎn)移率(57.8%)明顯高于皮下移植瘤模型(14.5%,P<0.01;37.1%,P<0.05)和腹腔內(nèi)移植瘤模型(18.0%,P<0.01;39.3%,P<0.05)。結(jié)論:人肝癌裸鼠皮下-肝原位移植瘤模型具有高存活率、高侵襲率和高轉(zhuǎn)移率的優(yōu)點,它的建立為研究人
3、類肝癌的轉(zhuǎn)移機制和篩選抗癌藥物提供了理想的工具。【關(guān)鍵詞】肝細胞瘤;移植,原位【中圖分類號】R735.7【文獻標(biāo)識碼】AEstablishmentoftheorthotopictransplantationtumormodelfromthesubcuta-neousmodelofhumanhepatocellularcarcinomainnudemiceZHENGJian-Ming,TAOWen-Zhao,ZHENGWei-Qiang,BAOJian-Zhong,WANGYan-Li,ZHUMing-Hua.(D
4、epartmentofPathology,ChanghaiHospital,SecondMilitaryMedicalUniversity,Shanghai200433,China)【ABSTRACT】Objective:Toestablishtheorthotopictransplantationtumormodelfromthesubcutaneousmodelofhumanhepatocellularcarcinomas(HCC)innudemice.Methods:FreshspecimensofHCCw
5、eretransplantedtoformsubcutaneoustransplantationtumorinnudemiceusinghistologicallyintacttissue.Thentheorthotopictransplantationtumormodelwases-tablishedbyinjectingthesubcutaneoustransplantedtumorintoliver(indirectorthotopicmodel).Itwascomparedwiththedirectort
6、hotopicmodel,subcutaneousmodelandperitonealcavitymodelforHCCinnudemice.Results:Theindirectortho-topicmodelpropagatedstably.Thesurvivalratesandspontaneousmetastaticratesofthetransplantationtumorwere100%and57.8%,respectively.Themodelhadhighersurvivalrates(100%)
7、thandirectorthotopicmodel(35.6%,P<0.01).Italsohadhigherlocalinvasionrates(100%)andspontaneousmetastaticrates(57.8%)thanthesubcutaneousmodel(14.5%,P<0.01;37.1%,P<0.05)andtheperitonealcavitymodel(18.0%,P<0.01;39.3%,P<0.05).Conclusion:Themodelisanidealinstrument
8、forstudyofthemetastaticmechanismandscreenofanti-metastaticdrugs.【KEYWORDS】hepatoma;transplantation,orthotopic[AcadJSecMilMedUniv,2000,21(5):456-459]自1969年在裸鼠體內(nèi)異種移植人類惡性腫瘤裸鼠肝內(nèi)原位移植瘤模型并對其生物學(xué)特