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1、FigureS1.miR-520fexpressionmodels.(A)pTet-onAdvancedandpmRi-ZsGreen1-miR-520fvectors.(B)LuciferaseactivityofT24-Tet-Onclone4cellsstimulatedwithdoxycycline(DOX,1μg/ml),comparedtonon-stimulatedcells(N=4).(C)FluorescentsignalofzsGreen1inT24-imiR-520fclone9cellsstimul
2、atedwithDOX,comparedtonon-stimulatedcells(N=2).(D)ExpressionanalysisofmiR-520finT24-imiR-520fclone9cellsstimulatedwithDOX,comparedtonon-stimulatedcells(N=4).(E)ExpressionanalysisofmiR-520finT24(N=4)andPANC-1(N=3)cellstransfectedwith20nMmiR-520fmimic,comparedtocont
3、rolmimictransfectedcells.*,p<0.05;**,p<0.01.(F)Phase-contrastimagesofT24andPANC-1cells3dayspost-transfectionwith20nMmiR-520fmimicorcontrolmimic(NC1),magnification40x.FigureS2.SOEingPCR.SchematicrepresentationofSOEingPCRsteps.FigureS3.ScreeningtoolsforEMT-reversing
4、miRNAs.(A)MapofthepEcad-Luc/Rlucreportervector.(B)PanelofcelllinesorderedbytheirepithelialormesenchymalphenotypebasedontheirCDH1/VIMexpressionratio.FigureS4.E-cadherinstainingofPANC-1cells.E-cadherinstainingofPANC-1cellstransfectedwith20nMmiR-520fmimicorwith20nMAD
5、AM9-specificsiRNA.Twodayspost-transfection,cellswerefixatedwith3%paraformaldehyde,andstainedwithanE-cadherin-specificantibody.Imagesweretakenat200xmagnification.A,Non-transfectedcells;B,NC1mimic;C,miR-520fmimic;D,controlsiRNA;E,siADAM9(I);F,siADAM9(II).Redarrowhea
6、dsindicatemembranousE-cadherinlocalization.FigureS5.3’-UTRreportervectors.(A)psiCHECK-2vectorcontainingtheADAM9orTGFBR23’-UTRregion.FigureS6.LeakymiR-520fexpressionandeffectofDOXonmetastasisformation.(A)ExpressionofmiR-520finlungtumors.*,p<0.05.(B)Percentageoftumo
7、rtissueinmouselungsectionsasassessedbyH&Estaining.(C)Bodyweightofmiceduringthestudy.
