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1、PROTOCOLElectricalstimulationsystemsforcardiactissueengineeringNinaTandon1,ChristopherCannizzaro2,Pen-HsiuGraceChao1,3,RobertMaidhof1,AnnaMarsano1,HoiTingHeidiAu4,MilicaRadisic4&GordanaVunjak-Novakovic11DepartmentofBiomedicalEngineering,622West168thStreet,VanderbiltClin
2、ic12th?oor,Room12-234,ColumbiaUniversity,NewYork,NewYork10032,USA.2DepartmentofBiomedicalEngineering,TuftsUniversity,4ColbyStreet,Medford,Massachusetts02155,USA.3InstituteofBiomedicalEngineering,NationalTaiwanUniversity,Zhanshulou503,1Sec.4,RooseveltRoad,Taipei10617,Tai
3、wan.4InstituteofBiomaterialsandBiomedicalEngineering,UniversityofToronto,164CollegeStreet,Room407,Toronto,OntarioM5S3G9,Canada.CorrespondenceshouldbeaddressedtoG.V.-N.(gv2131@columbia.edu).Publishedonline22January2009;doi:10.1038/nprot.2008.183Wedescribeaprotocolfortiss
4、ueengineeringofsynchronouslycontractilecardiacconstructsbyculturingcardiaccellswiththeapplicationofpulsatileelectrical?eldsdesignedtomimicthosepresentinthenativeheart.Tissuecultureisconductedinacustomizedchamberbuilttoallowforcultivationof(i)engineeredthree-dimensional(
5、3D)cardiactissueconstructs,(ii)cellnatureprotocols/monolayerson?atsubstratesor(iii)cellsonpatternedsubstrates.Thisalsoallowsforanalysisoftheindividualandinteractivemeffectsofpulsatileelectrical?eldstimulationandsubstratetopographyoncelldifferentiationandassembly.Theprot
6、ocolisoc.edesignedtoallowfordeliveryofpredictableelectrical?eldstimulitocells,monitoringenvironmentalparameters,andassessmentrutofcellandtissueresponses.Thedurationoftheprotocolis5dfortwo-dimensionalculturesand10dfor3Dcultures.an.www/INTRODUCTION/:ptTheoverarchinggoalof
7、cardiactissueengineeringistocreateinteractiveeffectsofsubstratetopographyandelectrical?eldthinvitrofunctionalcardiactissuethatcanbeusedasabiologicalstimulationincellmonolayers.pmodelforstudiesofcardiactissuedevelopmentand/ordiseaseTheheartisthebody’slargestbioelectrical
8、source11.Manyuoprocesses,andeventuallyasanimplanttorepairinjuredmyocar-groupshavebeenstudyingelectricalsignals
