資源描述：
《Differential Expression and Activity of Tissue-nonspecificAlkaline Phosphatase (TNAP) in Rat Odontogenic Cells In Vivo組織非特異性的差異表達與活性 Alkaline Phosphatase（TNAP）在大鼠牙源性細(xì)胞中的作用》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、Volume47(12):1541–1552,1999TheJournalofHistochemistry&Cytochemistryhttp://www.jhc.orgARTICLEDifferentialExpressionandActivityofTissue-nonspecificAlkalinePhosphatase(TNAP)inRatOdontogenicCellsInVivoDominiqueHotton,NicoleMauro,FrédéricLézot,NadineForest,andArianeBerdalLaborat
2、oiredeBiologie–Odontologie,InstitutBiomédicaldesCordeliers(DH,NM,FL,NF,AB),andINSERMU458(DH),Paris,FranceSUMMARYAmongthefourexistingisoformsofalkalinephosphatase(AP),thepresentstudyisdevotedtotissue-nonspecificalkalinephosphatase(TNAP)inmineralizeddentaltissues.Northernblot
3、analysisandmeasurementsofphosphohydrolaseactivityonmicro-dissectedepitheliumandectomesenchyme,insituhybridization,andimmunolabelingonincisorsconfirmedthattheAPactiveinrodentteethisTNAP.WhereasthedevelopmentalpatternofTNAPmRNAandproteinandthepreviouslydescribedactivityweresi
4、milarinsupra-ameloblasticandmesenchymalcells,theydifferedinenamel-secretingcells,theameloblasts.AspreviouslyshownforotherproteinsinvolvedincalciumandphosphateKEYWORDShandlinginameloblasts,abiphasicpatternofsteady-stateTNAPmRNAlevelswasassoci-alkalinephosphataseatedwithaddit
5、ionalvariationsinameloblastTNAPproteinlevelsduringthecyclicmodula-bonetionprocess.AlthoughtheassociationofTNAPupregulationandtheinitialphaseofbio-dentinmineralizationappearedtobeabasicfeatureofallmineralizedtissues,ameloblasts(andenameltoalesserextent,odontoblasts)showedase
6、condselectivelyprominentupregulationofmineralizationTNAPmRNAproteinactivityduringterminalgrowthoflargeenamelcrystalsonly,i.e.,thevitaminDmaturationstage.ThisdifferentialexpressionactivityforTNAPinteethvsbonemayex-hypophosphatasiaplainthestrikingdentalphenotypevsbonerepor
7、tedinhypophosphatasia,ahereditaryinsituhybridizationdisorderrelatedtoTNAPmutation.(JHistochemCytochem47:1541–1552,1999)immunolocalizationAlkalinephosphatases(APs)areubiquitousin1994;Whyteetal.1995).Inrodents,thecDNAsen-manyspecies,frombacteriatohuman(Manesetal.codingforthep
8、lacental,embryonic,andintestinal1990).Theiroveralldistributionindicatesthattheseis
