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《海蜇傘部不溶性膠原纖維及酶促溶性膠原蛋白的熱穩(wěn)定性對(duì)比研究》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、現(xiàn)代食品科技ModernFoodScienceandTechnology2016,Vol.32,No.6海蜇傘部不溶性膠原纖維及酶促溶性膠原蛋白的熱穩(wěn)定性對(duì)比研究11111,21,21,21,2王煦松,楊祺福,孫睿,張玉瑩,李冬梅,董秀萍,朱蓓薇,秦磊(1.大連工業(yè)大學(xué)食品學(xué)院,遼寧大連116034)(2.國(guó)家海洋食品工程技術(shù)研究中心,遼寧大連116034)摘要:海蜇(Rhopilemaesculentum)是我國(guó)傳統(tǒng)漁業(yè)生產(chǎn)的重要大型經(jīng)濟(jì)水母,其傘部膠原蛋白含量豐富,但膠原蛋白對(duì)熱敏感,對(duì)其熱穩(wěn)定性進(jìn)行研究對(duì)海蜇資源的開發(fā)利用至關(guān)重要。本論文從海蜇傘部提取得到不溶性膠原纖維
2、(ICF)及酶促溶性膠原蛋白(PSC),并對(duì)其熱穩(wěn)定性進(jìn)行對(duì)比分析。結(jié)果表明,海蜇傘部ICF與PSC的氨基酸組成及分子質(zhì)量類似于I型膠原蛋白,其高級(jí)結(jié)構(gòu)差異亦造成熱穩(wěn)定性的不同。傅里葉變換紅外光譜(FT-IR)研究發(fā)現(xiàn),在加熱至36℃時(shí),PSC的酰胺I帶紅外吸收驟然降低,結(jié)構(gòu)發(fā)生明顯變化,而ICF溫度升高至50℃時(shí),酰胺I帶紅外吸收才開始略有變化,ICF熱穩(wěn)定性優(yōu)于PSC。二維紅外分析研究發(fā)現(xiàn),PSC中310-螺旋結(jié)構(gòu)與ICF中β-折疊結(jié)構(gòu)(低頻率)對(duì)溫度變化最為敏感,除自動(dòng)峰外,PSC與ICF均存在若干交叉峰,說明熱處理過程中各個(gè)結(jié)構(gòu)之間存在著分子內(nèi)和分子間的相互作用。本研
3、究為海蜇膠原蛋白的開發(fā)利用提供了一定的理論基礎(chǔ)。關(guān)鍵詞:海蜇;不溶性膠原纖維;膠原蛋白;二維紅外光譜;熱穩(wěn)定性文章篇號(hào):1673-9078(2016)6-110-117DOI:10.13982/j.mfst.1673-9078.2016.6.018ThermalStabilityofInsolubleCollagenFibrilsandPepsin-SolubilizedCollagenfromRhopilemaesculentumUmbrellas11111,21,21,2WANGXu-song,YANGQi-fu,SUNRui,ZHANGYu-ying,LIDong-me
4、i,DONGXiu-ping,ZHUBei-wei,1,2QINLei(1.SchoolofFoodScienceandTechnology,DalianPolytechnicUniversity,Dalian116034,China)(2.NationalEngineeringResearchCenterofSeafood,Dalian116034,China)Abstract:Rhopilemaesculentum,alargejellyfish,isoneofthemosteconomicallyimportanttraditionalfisheryproductsin
5、China.Theumbrellasofjellyfisharerichincollagen.Sincecollagenistemperature-sensitive,itisimportanttostudyitsthermalstabilityforthedevelopmentandutilizationofjellyfishresources.Inthisstudy,insolublecollagenfibrils(ICFs)andpepsin-solubilizedcollagen(PSC)wereextractedfromumbrellasofRhopilemaesc
6、ulentum,andtheirthermostabilitywascompared.TheresultsindicatedthattheaminoacidcompositionsandmolecularweightsoftheICFandPSCweresimilartothoseoftypeIcollagen,andthedifferencesinthehigher-orderstructureofICFandPSCledtotheirdifferentthermalstabilities.Fouriertransforminfraredspectroscopy(FT-IR
7、)analysisshowedthattheintensityoftheamideIbandintheinfraredabsorptionofPSCdecreasedrapidly,andapparentstructuralchangesoccurredwhenthesamplewasheatedto36℃.Whenthetemperaturewasraisedto50℃,theintensityoftheamideIbandofICFchangedonlyslightly,suggestingthat