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1、石河子大學(xué)動物科技學(xué)院本科畢業(yè)論文墾區(qū)豬肺炎支原體P97基因的檢測院（系）：動物科技學(xué)院專業(yè)：動物醫(yī)學(xué)學(xué)號：2010513200姓名：張延浩指導(dǎo)教師：李劭（副教授）石河子2014.6為了解石河子墾區(qū)豬肺炎支原體P97基因的遺傳變異情況，采集石河子部分規(guī)?；i場的豬支原體肺炎疑似病料，采用PCR方法對疑似病料進行了黏附基因P97的檢測，將PCR產(chǎn)物測序并與Genbank已登錄的標(biāo)準(zhǔn)菌株序列進行同源性分析。結(jié)果顯示：75份病變肺臟中，陽性病料43份,陽性率為57.33%(43/75),P97基因序列與參考菌株的同源性為9
2、8.4%?99.2%。檢測結(jié)果表明石河子墾區(qū)豬支原體肺炎的感染情況比較嚴(yán)重，豬肺炎支原體流行株與國際標(biāo)準(zhǔn)菌株有很高的同源性。關(guān)鍵詞:豬肺炎支原體；P97基因；PCR檢測AbstractThecurrentsituationofprevalenceofMycoplasmahyopneumoniae(Mhp)anditscorrespondinggeneticvariationsofP97geneinsomelarge-scaledpigfarmsinthereclamationareaofShiheziwasmeasu
3、red?SuspiciousMhpsampleswerecollectedanddetectedthatPCR(polymerasechainreaction)methodwasutilizedtodetecttheP97whichisaadhesingeneofthepositiveMhpsamplesandtheobtainedgenesequencesofclinicsampleswerecomparedwiththestandardstrainssequenceintheGenbankviahomologya
4、nalysis.TheresultsshowthattheratioofpositivesamplesinallthedetectedsuspiciousMhplungsamplesis57.33%(43/75)andthegenetichomologyofthedetectedP97genesequencestothestandardstrainssequenceis98.4%?99.2%.OurdetectionresultsindicatethattheprevalenceofMhpinthereclamati
5、onareaofShihezianditssurroundingareasissevereandlocalepidemicMhpstrainshavehighhomologywithinternationalstandards.Key^vords:Mycoplasmahyopneum(miae;P91gene;PCRdetection1材料與方法.......................................................................................
6、..........................11.1材料...................................................................................................................11.1.1樣品來源........................................................................................................
7、11.1?2主要試劑....................................................................................................11.1.3主要儀器........................................................................................................11.2方法...............................
8、....................................................................................21.2.1病料組織DNA的提取.........................................................................
